Figure 5. Inhibition of Glucose Utilization in Poly(I:C)-Induced Inflammation Enhances ER Stress
(A) WT andIfnar/mice were challenged with poly(I:C) and then treated with either IP PBS or 2DG. Whole-hindbrain lysates 24 hr after poly(I:C) treatment
immunoblotted for CHOP andb-tubulin are shown.
(B) Hindbrain mRNA expression ofGadd3418 hr after poly(I:C) challenge and treatment with IP PBS or 2DG in WT mice. n = 5/group (one death in poly(I:C) group).
(C) Survival of WT mice andDdit3/mice after poly(I:C) challenge and treatment with IP PBS or 2DG. WT: poly(I:C) + 2DG versusDdit3/: poly(I:C) + 2DG
p = 0.0015. n = 5/group.
(D and E) WT andDdit3/mice were challenged with poly(I:C) and treated with 2DG. (D) Plasma IFNais shown. n = 5/group. (E) Vital signs measured 18 hr after
poly(I:C) + 2DG are shown. n = 3–7/group (vital sign values of WT mice same as those inFigure 4D).
(F–H) WT mice andDdit3/mice were infected with 700 PFUs of influenza virus and treated with 2DG. (F) Survival after influenza infection is shown. p = 0.048;
n = 5/group. (G) Plasma IFNais shown. n = 5/group. (H) Lung and BAL viral load 5 days post-infection is shown. n = 5/group.
Data are represented as mean±SEM. **p < 0.01; ***p < 0.001. See alsoFigure S5.
Cell 166 , 1512–1525, September 8, 2016 1519