Supplemental Figures
Figure S1. CPR5 Is a Transmembrane Protein Enriched in the Nuclear Pore, Related toFigure 1
(A) Leaf epidermal cells of two-week-old35S:GFP-CPR5/cpr5-1transgenicArabidopsisseedlings were imaged using confocal laser scanning microscopy.
Three-dimensional image of the nuclear surface was reconstructed from z stack images using Imaris (right). Arrowheads indicate the nuclear envelope (NE) or the
nucleus (N).
(B) Co-localization of GFP-CPR5 with endomembrane markers. GFP-CPR5 was transiently co-expressed with mCherry-tagged endomembrane markers in
N. benthamiana. The nuclear envelope (NE) marker is mCherry-WIT1. TGN/EE,trans-Golgi network/early endosome. MVB/LE, multi-vesicular body/late
endosome.
(C) Peptide coverage of the carboxyl terminal half of the AtCPR5 protein. GFP-CPR5 was affinity purified from transgenic plants and subject to LC-MS/MS. The
predicted transmembrane domains in CPR5 are labeled in red. Predicted omission regions by trypsin digestion are labeled in cyan. Individual peptides detected
by LC-MS/MS are labeled in brown and listed below the reference sequence.