Cell - 8 September 2016

Figure S4. CPR5 Modulates Nucleocytoplasmic Transport of Stress-Related Cargo, Related toFigure 4
(A) Transient coexpression of mCherry-tagged NPR1/JAZ1/ABI5 with YFP-tagged WT (left panel) or G420D mutant (right panel) CPR5 inN. benthamiana.
(B) Quantification of results from experiments performed in (A). Localization of nuclear proteins in each cell was recorded as binomial data and categorized as
‘nucleus only’ or with significant ‘cytosol retention’. A logistic regression model using CPR5/G420D as the sole independent variable can explain 95% of the data
variance, illustrating the predominant effect of the functional CPR5 on protein nuclear transport.
(C) Phenotypes observed whencpr5was crossed with stress-related karyopherin and transport-related nucleoporin mutants.