CD
hsp-16.2p::GFP (with hsp-6 RNAi)
Control Brain CM C16 C20 C22 C24
hsp-16.2p::GFP
Control Heart CL C14 C18
A
Control(EV) EV: hsp-6 hsp-6: crls-1
Nonyl Acridine Orange Staining
RNAi
B
Control(EV) EV: hsp-6 hsp-6: crls-1
hsp-16.2p::GFP
ppppppppppppppp p RNAi
E
Sphingomyelin T10B11.2
Ceramide-1-phosphate
Serine + Palmitoyl CoA
Sphinganine
3-Ketosphinganine
Dihydroceramide
Ceramide
Sphingosine
sptl-1,2,3
hyl-1,2 and lagr-1
asm-1,2,3
Ceramidase
Cardiolipin W02F12.2, F27E5.1, K11D2.2
Cardiolipin
F
RNAi hsp-16.2p::GFP
induction
hsp-16.2p ::GFP suppression
(Double RNAi with hsp- 6
RNAi)
sptl-1 Yes n/a
sptl-2 Yes n/a
sptl-3 Yes n/a
hyl-1 Yes n/a
hyl-2 Yes n/a
lagr-1 No n/a
asm-2 Yes n/a
asm-3 Yes n/a
ZK856.18 (asm-2 ortholog) Yes n/a
T10B11.2 No Yes
W02F12.2 No Yes
F27E5.1 No Yes
K11D2.2 No Yes
0
5000
10000
15000
20000
25000
30000
35000
40000
EV sptl-1 sptl-2 sptl-3 hyl-1 hyl-2 lagr-1
asm-2 ZK856.18 asm-3
Peak GFP Fluorescence (RFU)
hsp-16.2p::GFP (peak)
***
*
*
* *
** ****
**
0
100
200
300
400
500
600
700
800
EV T10B11.2 K11D2.2 F27E5.1 W02F12.2
GFP Fluorescence (RFU)
hsp-16.2p::GFP (double RNAi)
***
**
** **
Double RNAi with hsp-6 RNAi
(legend on next page)
1546 Cell 166 , 1539–1552, September 8, 2016