STAR+METHODS
KEY RESOURCES TABLE
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
NDUFS3 Abcam Ab14711; RRID: AB_301429
mtHSP70 Abcam Ab82591; RRID: AB_1860633
Alpha-Tubulin Sigma-Aldrich T6074, RRID:AB_477582
GFP Roche 11814460001; RRID: AB_390913
Cytosolic HSP70/HSP72 Enzo life ADI-SPA-810-D; RRID: AB_2039260
HSP60 University of Iowa-
Developmental Studies
Hybridoma Bank
HSP60Chemicals, Peptides, and Recombinant Proteins
Perhexiline Sigma-Aldrich SML-0120
Nile Red Sigma-Aldrich N3013
Nonyl Acridine Orange Invitrogen A-1372
Ceramides (C16) Avanti Polar Lipids, Inc. 860516P
Ceramide (C20) Avanti Polar Lipids, Inc. 860520P
Ceramide (C22) Avanti Polar Lipids, Inc. 860501P
Ceramide (C24) Avanti Polar Lipids, Inc. 860524P
Natural Ceramide Avanti Polar Lipids, Inc. 860052P
Cardiolipins (C14) Avanti Polar Lipids, Inc. 750332P
Cardiolipin (C18) Avanti Polar Lipids, Inc. 710335P
Natural Cardiolipin Avanti Polar Lipids, Inc. 840012P
Critical Commercial Assays
Triglyceride quantification Kit Bio Vision K622-100
Deposited Data
Raw and analyzed microarray data This paper GEO: GSE83722
Experimental Models: Cell Lines
Human: HEK293 cells Life Technologies R70007
Human: primary normal human dermal
fibroblasts (NHDF)
Lonza CC-2511Experimental Models: Organisms/Strains
C. elegans: SJ4100 (zcIs13[hsp-6p::GFP]) Caenorhabditis Genetics
Center (CGC)
N/AC. elegans: SJ4058 (zcIs9[hsp-60p::GFP]) CGC N/A
C. elegans: CL2070 (dvIs[hsp-16.2p::GFP]) CGC N/A
C. elegans: CF512 (fer-15(b26);fem-1(hc17)) CGC N/A
C. elegans: AM140 (rmls132[unc-54p::Q35::YFP]) CGC N/A
C. elegans: SJ4197 (zcIs39 [dve-1p::dve-1::GFP]) CGC N/A
C. elegans: AGD710 (N2, uthls235 [sur-5p::hsf-1,
myo-2p::tomato]
(Baird et al., 2014) N/AC. elegans: N2 CGC N/A
C. elegans: AGD919 (dvIs[hsp-16.2p::GFP];
fer-15(b26);fem-1(hc17))
This paper N/ARecombinant DNA
pLenti6.3/V5-DEST Proteostasis Therapeutics N/A
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