Sample Triglyceride control (C)=B/VXDnmol/mlormM
Where:Bis the amount of triglyceride from standard curve (nmol)
Vis the sample volume added into the reaction well (ml)
Dis the sample dilution factorFor the mammalian cell culture samples, cells were washed with cold PBS and homogenized with 5% NP-40 in water using a
syringe with 22G needle, followed by the procedure shown above. All experiments were done with three biological repeats.
Quantification
Western blots were quantified using the ImageJ software.
GFP reporter expression was quantified using the COPAS Biosorter. At least 300 adult worms were used for each biological
sample. Larvae or broken worms were excluded by filtering the extinction and TOF from raw data.
Statistics
Statistical parameters, including the exact value of n and descriptive statistics (mean±SD) and statistical significance are reported in
the method details, figures and the figure legends: Number of worms used for analyses are reported in the method details. Mean of
three independent biological replicates were shown with standard deviation (SD) and P values were calculated using a standard
Student’s t test. Statistical significance is indicated (: p < 0.05, : p < 0.01, : p < 0.0001) in the figures and figure legends.
Lipidomic analysis was performed with five independent biological replicates and mean values were shown with standard mean of
the error (mean±SEM).
DATA AND SOFTWARE AVAILABILITY
The accession number for the microarray data reported in this paper is NCBI GEO: GSE83722.
e6 Cell 166 , 1539–1552.e1–e6, September 8, 2016