promotes stress granule assembly and drives pathological fibrillization. Cell
163 , 123–133.
Osborne Nishimura, E., Zhang, J.C., Werts, A.D., Goldstein, B., and Lieb, J.D.
(2015). Asymmetric transcript discovery by RNA-seq in C. elegans blasto-
meres identifies neg-1, a gene important for anterior morphogenesis. PLoS
Genet. 11 , e1005117.
Pagano, J.M., Farley, B.M., McCoig, L.M., and Ryder, S.P. (2007). Molecular
basis of RNA recognition by the embryonic polarity determinant MEX-5.
J. Biol. Chem. 282 , 8883–8894.
Poser, I., Sarov, M., Hutchins, J.R.A., He ́riche ́, J.-K., Toyoda, Y., Pozniakov-
sky, A., Weigl, D., Nitzsche, A., Hegemann, B., Bird, A.W., et al. (2008). BAC
TransgeneOmics: a high-throughput method for exploration of protein function
in mammals. Nat Methods 5 , 409–415.
Rizk, A., Paul, G., Incardona, P., Bugarski, M., Mansouri, M., Niemann, A., Zie-
gler, U., Berger, P., and Sbalzarini, I.F. (2014). Segmentation and quantification
of subcellular structures in fluorescence microscopy images using Squassh.
Nat. Protoc. 9 , 586–596.
Rubinstein, M., and Colby, R.H. (2003). Polymer Physics (Oxford University
Press).
Schisa, J.A., Pitt, J.N., and Priess, J.R. (2001). Analysis of RNA associated with
P granules in germ cells ofC. elegansadults. Development 128 , 1287–1298.
Schubert, C.M., Lin, R., de Vries, C.J., Plasterk, R.H., and Priess, J.R. (2000).
MEX-5 and MEX-6 function to establish soma/germline asymmetry in early
C. elegansembryos. Mol. Cell 5 , 671–682.
Schwartz, J.C., Wang, X., Podell, E.R., and Cech, T.R. (2013). RNA seeds
higher-order assembly of FUS protein. Cell Rep. 5 , 918–925.
Seydoux, G., and Fire, A. (1994). Soma-germline asymmetry in the
distributions of embryonic RNAs inCaenorhabditis elegans. Development
120 , 2823–2834.
Sheth, U., Pitt, J., Dennis, S., and Priess, J.R. (2010). Perinuclear P granules
are the principal sites of mRNA export in adultC. elegansgerm cells. Develop-
ment 137 , 1305–1314.
Spike, C.A., Bader, J., Reinke, V., and Strome, S. (2008). DEPS-1 promotes
P-granule assembly and RNA interference inC. elegansgerm cells. Develop-
ment 135 , 983–993.
Strome, S., and Wood, W.B. (1982). Immunofluorescence visualization
of germ-line-specific cytoplasmic granules in embryos, larvae, and adults of
Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 79 , 1558–1562.
Strzelecka, M., Trowitzsch, S., Weber, G., Lu ̈hrmann, R., Oates, A.C., and
Neugebauer, K.M. (2010). Coilin-dependent snRNP assembly is essential for
zebrafish embryogenesis. Nat. Struct. Mol. Biol. 17 , 403–409.
Su, X., Ditlev, J.A., Hui, E., Xing, W., Banjade, S., Okrut, J., King, D.S., Taunton,
J., Rosen, M.K., and Vale, R.D. (2016). Phase separation of signaling mole-
cules promotes T cell receptor signal transduction. Science 352 , 595–599.
Tenlen, J.R., Molk, J.N., London, N., Page, B.D., and Priess, J.R. (2008).
MEX-5 asymmetry in one-cellC. elegansembryos requires PAR-4- and
PAR-1-dependent phosphorylation. Development 135 , 3665–3675.
Thandapani, P., O’Connor, T.R., Bailey, T.L., and Richard, S. (2013). Defining
the RGG/RG motif. Mol. Cell 50 , 613–623.
Tintori, S.C., Osborne Nishimura, E., Golden, P., Lieb, J.D., and Goldstein, B.
(2016). A transcriptional lineage of the earlyC. elegansembryo. bioRxiv. Pub-
lished online April 8, 2016.http://dx.doi.org/10.1101/047746.
Tyanova, S., Temu, T., Sinitcyn, P., Carlson, A., Hein, M.Y., Geiger, T., Mann,
M., and Cox, J. (2016). The Perseus computational platform for comprehen-
sive analysis of (prote)omics data. Nat Methods. Published online June 27,
2016.http://dx.doi.org/10.1038/nmeth.3901.
Updike, D., and Strome, S. (2010). P granule assembly and function in
Caenorhabditis elegansgerm cells. J. Androl. 31 , 53–60.
Updike, D.L., Hachey, S.J., Kreher, J., and Strome, S. (2011). P granules
extend the nuclear pore complex environment in theC. elegansgerm line.
J. Cell Biol. 192 , 939–948.
Updike, D.L., Knutson, A.K., Egelhofer, T.A., Campbell, A.C., and Strome, S.
(2014). Germ-granule components prevent somatic development in the
C. elegansgermline. Curr. Biol. 24 , 970–975.
Voronina, E. (2013). The diverse functions of germline P-granules inCaeno-
rhabditis elegans. Mol. Reprod. Dev. 80 , 624–631.
Wang, J.T., Smith, J., Chen, B.-C., Schmidt, H., Rasoloson, D., Paix, A., Lamb-
rus, B.G., Calidas, D., Betzig, E., and Seydoux, G. (2014). Regulation of RNA
granule dynamics by phosphorylation of serine-rich, intrinsically disordered
proteins inC. elegans. eLife 3 , e04591.
Wang, X., Schwartz, J.C., and Cech, T.R. (2015). Nucleic acid-binding speci-
ficity of human FUS protein. Nucleic Acids Res. 43 , 7535–7543.
Wippich, F., Bodenmiller, B., Trajkovska, M.G., Wanka, S., Aebersold, R., and
Pelkmans, L. (2013). Dual specificity kinase DYRK3 couples stress granule
condensation/dissolution to mTORC1 signaling. Cell 152 , 791–805.
Wisniewski, J.R., Hein, M.Y., Cox, J., and Mann, M. (2014). A ‘‘proteomic ruler’’
for protein copy number and concentration estimation without spike-in stan-
dards. Mol. Cell. Proteomics 13 , 3497–3506.
Wu, Y., Zhang, H., and Griffin, E.E. (2015). Coupling between cytoplasmic con-
centration gradients through local control of protein mobility in theCaenorhab-
ditis eleganszygote. Mol. Biol. Cell 26 , 2963–2970.
Zhang, H., Elbaum-Garfinkle, S., Langdon, E.M., Taylor, N., Occhipinti, P.,
Bridges, A.A., Brangwynne, C.P., and Gladfelter, A.S. (2015). RNA controls
PolyQ protein phase transitions. Mol. Cell 60 , 220–230.1584 Cell 166 , 1572–1584, September 8, 2016