Cell - 8 September 2016

Figure S2. Contribution of Different Structural Features of RNA to PGL-3 Drop Assembly, Related toFigure 3
(A–D) Maximum intensity projections of series of confocal z- slices show assembly of drops of PGL-3-mEGFP (0.6mM) on addition of different kinds of RNA.
(A) No RNA, totalC. elegansmRNA (10 ng/mL), in vitro transcribed luciferase mRNA lacking 5^0 cap and poly(A) tail (10 ng/mL), in vitro transcribed 18SC. elegans
rRNA (10 ng/mL), total RNA fromC. elegans(10 or 200 ng/mL). The micrograph with totalC. elegansmRNA is identical as inFigure 3B.
(B) Luciferase mRNA fragments 200, 400, 600 or 800 bases long (20 nM), lacking 5^0 cap and poly(A) tail.
(C) Drop assembly on addition of in vitro transcribed 18S rRNA pre-heated at 75C for 1 min. no RNA (control buffer pre-heated), 18S rRNA (40 nM) with or without
pre-heating.
(D) In vitro transcribed 18SC. elegansrRNA (50 or 200 ng/mL).
(E) Single confocal plane micrograph show drops of RGG_mut-mEGFP at 10mM in absence of RNA.