Cell - 8 September 2016

Figure S3. Example smFISH and RNA-Seq Analysis to Quantify mRNA inC.elegansEmbryos, Related toFigure 2
(A) Example smFISH and RNaseq analysis to quantifytes-1mRNA. smFISH quantitates individual mRNA molecules for specific transcripts using tiled fluo-
rescently labeled probes on fixed samples.tes-1transcripts shown in green, and DNA is stained in DAPI in 2-cellC. elegansembryo. Single-cell resolution
RNaseq assesses relative levels of mRNA molecules globally for each cell.