Figure S5. Characterization of Binding between PGL-3 andC.elegansRNA, Related toFigure 5
(A–C) qPCR analysis of RNA that co-immunoprecipitated with PGL-3-mEGFP orDRGG-mEGFP from a pool of total RNA purified fromC. elegansgermline.
(A) Immunoblot with an anti-GFP antibody to compare the IP efficiency of PGL-3-mEGFP (FL) andDRGG-mEGFP (DRGG). Equal amounts of IP-ed material were
loaded.
(B) Plot of relative RNA enrichment of ten different mRNA species and the 26S and 18S rRNA. Relative RNA enrichment describes the ratio of the amounts ofa
particular RNA species co-IPed with PGL-3 overDRGG.
(C) Plot shows that the amounts of co-IPed mRNA do not correlate with the lengths of these mRNA. r is Pearson coefficient related to the linear fit.
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