Cell - 8 September 2016

Article

Structure of a Complete Mediator-RNA Polymerase II

Pre-Initiation Complex

Graphical Abstract

Highlights

d Isolation of a 52-protein, 2.5 MDa Mediator-RNA polymerase

II initiation complex

d Structural elucidation of the complex by cryo-EM and

chemical cross-linking

d Model for the regulation of CTD phosphorylation during

transcription initiation

d Mediator Tail module architecture and dynamics

Authors

Philip J. Robinson, Michael J. Trnka,

David A. Bushnell, Ralph E. Davis,

Pierre-Jean Mattei, Alma L. Burlingame,

Roger D. Kornberg

Correspondence

[email protected]

In Brief

Visualizing a complete Mediator-RNA

polymerase II pre-initiation complex

suggests a model explaining the basis for

control of transcription initiation by the

pol II C-terminal domain.

Robinson et al., 2016, Cell 166 , 1411–1422

September 8, 2016ª2016 Elsevier Inc.

http://dx.doi.org/10.1016/j.cell.2016.08.050