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ACKNOWLEDGMENTS
We thank all members of the Lüthi group for helpful discussions
and comments on the work; G. Keller, D. Gerosa Erni, and T. Lu for
virus production; P. Argast, C. Mueller, T. Eichlisberger, and all staff of
the FMI Animal Facility for excellent technical assistance; J. Eglinger,
S. Bourke, and R. Thierry at the FMI imaging facility and the FMI
IT department for their dedicated technical support; and
V. Jayaraman, R. Kerr, D. Kim, L. Looger, K. Svoboda, and the HHMI
Janelia GENIE Project for making GCaMP6 available.Funding:This
work was supported by the Novartis Research Foundation, the
European Research Council (ERC) under the European Union’s
Horizon 2020 Research and Innovation Programme (grant
agreements 669582 to A.L. and 803870 to J.G.), the National Center
of Competences in Research:“SYNAPSY—The Synaptic Bases of
Mental Diseases”(financed by the Swiss National Science Foundation
SNSF, 51NF40-158776), SNSF core (310030B_170268; all to A.L.),
and SNSF Professorship (PP00P3_170672 to JG) grants; as well as a
Young Investigator Grant from the Brain & Behavior Research
Foundation (23593), a Career Development Award from the Synapsis
Foundation–Alzheimer Research Switzerland ARS (2018-CDA02),
and the Fondazione HED ALBIREO (to S.K.). A.S. was supported by a
postdoctoral fellowship from the European Molecular Biology
Organization (EMBO-ALTF-233-2020).Author contributions:S.D.,
S.K, J.G., and A.L. designed the project. S.D. and A.S. performed
imaging experiments. S.D. performed imaging data analysis. M.M.
performed in vitro patch-clamp experiments. S.D. and S.K. designed,
generated, and tested the viral constructs. All authors contributed
to the experimental design and interpretation of the data and wrote
the manuscript.Competing interests:The authors declare no
competing interests.Data and materials availability:All data and
analyses necessary to understand and assess the conclusions ofthe manuscript are presented in the main text and in the
supplementary materials. All processed data and scripts needed
to evaluate the conclusions in the paper are available athttps://data.
fmi.ch/PublicationSupplementRepowith no access restriction.SUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abf7052
Figs. S1 to S11
Tables S1 and S2
References ( 49 – 58 )
MDAR Reproducibility Checklist
Movies S1 and S213 November 2020; resubmitted 6 January 2022
Accepted 10 March 2022
10.1126/science.abf7052dÕAquinet al.,Science 376 , eabf7052 (2022) 15 April 2022 13 of 13
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