Stem Cell Processing (Stem Cells in Clinical Applications)

172 lic and privately owned UCB SCBs have been established that are actively involved around the world in collecting, processing ...

173 Developing countries often have to prioritize providing basic healthcare needs to their populations while also addressing ep ...

174 transplants have been performed to date, and there are over 700,000 and four mil- lion units stored globally in public and p ...

Table 8.2 Number of registered clinical studies using UCB and UC-derived cells for non- hematopoietic and regenerative medicine ...

176 The treatment of neurological diseases is by far the most active area of research (Iafolla et al. 2014 ). The rationale for ...

177 based on rational principles will be essential. Notably, each and every indication and cell therapy will require specifi c c ...

178 Bensussan A, Gluckman E, el Marsafy S et al (1994) BY55 monoclonal antibody delineates within human cord blood and bone marr ...

179 Kanda Y, Kanda J, Atsuta Y et al (2014) Changes in the clinical impact of high-risk human leuko- cyte antigen allele mismatc ...

180 Wang F, Maeda N, Yasuhara T et al (2012) The therapeutic potential of human umbilical cord blood transplantation for neonata ...

© Springer International Publishing Switzerland 2016 181 P.V. Pham (ed.), Stem Cell Processing, Stem Cells in Clinical Applicati ...

182 be used to carry out more accurate drug discovery and toxicity studies on human models (Rubin 2008 ). Stem cells help to ove ...

183 they have increased expression of a gene called telomerase. High levels of telomer- ase maintain the telomere ends of the ch ...

184 in vitro and in vivo (Chiu and Rao 2003 ), they cannot give rise to extraembryonic tissues such as the placenta and membrane ...

185 antiserum followed by separate exposure to complement (Solter and Knowles 1975 ). However, immunosurgery uses substances, li ...

186 9.2.3 Culture Traditionally, human ES cells were cultured on mouse embryonic fi broblast (MEF) feeder layers, which maintain ...

187 feeders and a serum-free medium, defi ning a totally animal-free culture system (Amit et al. 2003 ). Further, Lee et al. ( 2 ...

188 (Odorico et al. 2001 ). Later, clonally derived human ES cells were produced, as reported by Amit et al. ( 2000 ), that demo ...

189 specifi c factors, but this requires better understanding of the events regulating cell lineage commitment and differentiati ...

190 Even after successful isolation and expansion, the major obstacle of differentia- tion into the specifi c cell type remains, ...

191 9.4 Overcoming Challenges 9.4.1 Somatic Cell Nuclear Transfer The two major challenges faced with the use of pluripotent hum ...