Stem Cell Processing (Stem Cells in Clinical Applications)

192 9.4.2 Induced Pluripotent Stem (iPS) Cells Further, pluripotent cells exhibiting similar functionality to ES cells can be di ...

193 successfully generated ES-like cell lines from mouse embryonic fi broblasts and skin fi broblasts by simply expressing four ...

194 et al. ( 2014 ), a bioactive scaffold capable of mediating cell differentiation and for- mation of an extracellular matrix t ...

195 9.4.5 Pro-survival Molecules Work with ES cells is limited as they are very diffi cult to culture and usually die when stem ...

196 patients within limited time will require extensive research. A better approach would be to obtain and store allogeneic ES c ...

197 Lo B, Parham L (2009) Ethical issues in stem cell research. Endocr Rev 30(3):204–213. http:// press.endocrine.org/doi/full/1 ...

© Springer International Publishing Switzerland 2016 199 P.V. Pham (ed.), Stem Cell Processing, Stem Cells in Clinical Applicati ...

200 isolation reports in the literature, protocols for the isolation of cells from these tissues and in particular from adipose ...

201 and cytokine source for osteogenic and hematopoietic events. During lactation, energy and some nutrients are provided by mam ...

202 Loss of function studies has indicated that PPAR-γ is required for adipogenesis both in vitro and in vivo. Thiazolidinedione ...

203 10.2.2 Patient Age and Gender Marked differences have been observed between genders in both the metabolism and endocrine fun ...

204 doublings at higher seeding densities were not statistically signifi cant. The older patients however had a smaller regressi ...

205 cultures, isolated both at 1 h and at 24 h. In addition, these results clearly demonstrated that adipose tissue extraction b ...

206 10.2.3.2 Background on Liposuction The surgical technique of removing fat through a small incision using suction was develop ...

207 The introduction of a syringe instead of a machine to aspirate the fat was introduced by Fournier and later optimized by Col ...

208 cell viability assessments that there was no substantial difference in cell phenotype using wet and dry liposuction techniqu ...

209 The harvesting cannula is inserted through the puncture wound into the subcuta- neous adipose layer of the abdominal donor s ...

210 Fig. 10.2 A puncture wound made with a no. 15 scalpel blade in an aseptically cleaned donor area. The harvesting cannula is ...

211 blood contaminants appear in the syringe. This method is successful in obtaining large volumes of ASCs from the SVF and does ...

212 for direct comparison of reported biological properties and experimental outcomes, especially in the context of cell-based t ...