Stem Cell Processing (Stem Cells in Clinical Applications)

151 tissue previously recorded. The ratio of compacted adipose tissue volume to the volume of collagen solution should be at lea ...

152 80–90 % of the culture flask surface area. In order to determine the volume of cell suspension required for reseeding after ...

153 the counting beads and debris. The counting beads are enumerated in the CAL region. In order to obtain an accurate absolute ...

154 Qualitative Assessment of Adipogenesis In Vitro Using Oil Red O After 21 days of induction, the 6-well plates containing bot ...

155 Induction of Osteogenesis In Vitro Immunophenotyped ASCs are seeded at a density of 5 × 10^3 cells/cm^2 in a six-well plate ...

156 Qualitative Assessment of Chondrogenesis In Vitro Each induced and non-induced chondrocyte sphere is serially dehydrated in ...

157 Bieback K, Hecker A, Schlechter T et al (2012) Replicative aging and differentiation potential of human adipose tissue-deriv ...

158 Doucet C, Ernou I, Zhang Y et al (2005) Platelet lysates promote mesenchymal stem cell expan- sion: a safety substitute for ...

159 Jeon ES, Song HY, Kim MR et al (2006) Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mese ...

16 0 Müller I, Kordowich S, Holzwarth C et al (2006) Animal serum-free culture conditions for isola- tion and expansion of multi ...

161 Sotiropoulou PA, Perez SA, Salagianni M et al (2006) Characterization of the optimal culture conditions for clinical scale p ...

© Springer International Publishing Switzerland 2016 163 P.V. Pham (ed.), Stem Cell Processing, Stem Cells in Clinical Applicati ...

16 4 8.1 Introduction The potential use of umbilical cord blood (UCB) was fi rst proposed in 1982 by Edward Boyse, whereafter th ...

165 transplants that mismatch at one or two alleles compared to a fully HLA-matched transplant (Kanda et al. 2014 ). Two distinc ...

166 In addition to its use in transplantation, UCB is a valuable source of cells for cel- lular therapies associated with tissue ...

167 Table 8.1 Benefi ts and limitations of UCB SCBs (Abdullah 2011 ; Ballen et al .^2008 ; Butler and Menitove 2011 ; Guilcher e ...

168 include the collection, testing, and processing of the units for storage, the preservation of the UCB units, and the man pow ...

169 (Sullivan 2008 ). This is because the current applications of stem cell therapy are lim- ited mainly to HSCT, for which the ...

170 8.1.2.3 Hybrid Cord Blood Banks Hybrid UCB SCBs are an amalgamation of public and private UCB SCBs (Fig. 8.1 ). In this sett ...

171 a bank is to be directed for later personal or family use, because most condi- tions that might be helped by UCB stem cells ...