117tissue and use thereof and isolation of stromal vascular fraction from vascular tis-
sues (IntelliCell BioSciences Inc., USA), Cell Extraction Easy Kit (Regenmedlab
Co., Ltd., Ho Chi Minh City, Vietnam); nonenzymatic method for isolating human
adipose-derived stromal/stem cells (Pennington Biomedical Research Center,
USA), isolation of stem cells from adipose tissue by ultrasonic cavitation, and meth-
ods of use (Rusty Property Holdings Pty. Ltd., Australia/Amberdale Enterprises Pty.
Ltd., Australia/Tavid Pty., Australia), and selective lysing of cells using ultrasound
(Solta Medical, Inc., USA) (Fig. 6.5 ; Table 6.6 ).
6.6 Ex Vivo-Expanded MSCs
6.6.1 Media
Popular media for MSC expansion are α -minimal essential medium or Dulbecco’s
modifi ed Eagle’s medium (DMEM) supplemented with 10 % fetal calf serum FCS.
In recent studies, DMEM/F12 (1:1) has been used as basal medium for MSCs,
Fig. 6.5 Commercialized kits for ADSC isolation. ( a ) Sepax 2, ( b ) Celution, ( c ) StromaCell, ( d )
Lipogems, ( e ) HuriCell, ( f ) MyStem
6 Production of Clinical-Grade Mesenchymal Stem Cells