Stem Cell Processing (Stem Cells in Clinical Applications)

(Michael S) #1

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Table 6.5 Kits and isolation systems for adipose tissue based on enzymes currently patented,
published, or commercially available


No. Company Device/method
1 ED Co. Ltd. and Pure Biotech Co.,
Ltd., South Korea/Medica Group,
United Arab Emirates

Sceldis ®

2 Cytori Therapeutics, Inc., USA Celution ® 800/CRS and 820/CRS
3 Cellular Biomedicine Group, Inc./
Cellular Biomedicine Group HK,
Ltd., USA

A-Stromal™ Kit

4 Cellthera, s.r.o., Czech Republic Cellthera Kits I and II and Method
for isolation of adipose tissue-
derived stromal vascular fraction
5 Biosafe Group SA, Switzerland Sepax 2
6 AdiStem Pty. Ltd., China AdiStem™ Small/Large Kit and
AdiLight
7 General Electric Company, USA Automated systems and methods
for isolating regenerative cells
from adipose tissue
8 GID Group, Inc., USA GID SVF-1™
9 Hurim BioCell, Co., Ltd., South
Korea

HuriCell

10 Medikan International Inc., USA STEM-X™
11 Ingeneron, Inc., USA Apparatus and methods for cell
isolation
12 N-BIOTEK, Inc., South Korea Beauty Cell
13 NeoGenesis Co., Ltd., South Korea UNISTATION™
14 GeneWorld Co., Ltd, Vietnam ADSC Extraction Kit
15 PNC International Co., Ltd., South
Korea/PNC North America Division
Of Advanced Bio-Medical
Equipment Co., Inc.

CHA STATION™ and
Multi-Station

16 SNJ Co., Ltd., South Korea/
TOPMED CO., LTD., South Korea

CID300

16 Regenmedlab Co., Ltd, Vietnam Cell Extraction Kit
17 Stempeutics Research Pvt. Ltd., India Stempeutron™

except for MSCs from umbilical cord blood, which use Iscove’s modifi ed Dulbecco’s
medium. Using these media, MSCs can be grown but with a long doubling time
(more than 1 month) to achieve useful quantities. To stimulate MSC growth and
reduce the doubling time, cytokines or growth factors (GFs) are added to the
medium. Three GFs are commonly used: epidermal growth factor (EGF), basic
fi broblast growth factor (FGF), and platelet-derived growth factor (PDGF) (Tarte
et al. 2010 ). Although these media are simple, inexpensive, and convenient for
ex vivo MSC culture, they contain a xenogeneic source of GFs and proteins (from


P.V. Pham and N.B. Vu
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