Fig. 7.4 Flow cytometric analysis of adipocyte differentiation of ASCs after staining with Nile
Red (20 ng/ml final concentration). Adipocyte differentiation is associated with the intracellular
accumulation with lipid droplets, which consequently results in an increase in cellular complexity.
Green-coloured events indicate early stages of intracellular lipid accumulation. Orange-coloured
events are indicative of cells with higher levels of intracellular neutral lipid content due to the
accumulation of intracellular lipid droplets (increase in cellular complexity). Flow cytometric
analysis was performed on a Beckman Coulter 3-laser, 10-colour Gallios flow cytometer, after 21
days of incubation of ASCs in adipogenic medium
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(Michael S)
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