269Fig. 16.4 Schematic representation of some culture parameters of the
fermentative strain BS2 isolated from Lake Pavin. (a) Evolution of
Fe(II) concentrations (mM) in glucose + iron (III) oxide-hydroxide
(G + HFO) medium. (b) Evolution of cell densities (cell.mL−1) in glu-
cose (G) and G + HFO media. (c) Evolution of glucose concentrations
(mM) in G and G + HFO media. (d) Thermograms (thermal power-time
curves) of BS2 grown anaerobically in G or in G + HFO media mea-
sured using microcalorimetry (Box 16.2). (e) Transmission electron
micrographs of BS2 strain in G + HFO medium at the beginning of the
growth phase (T0) showing the attachment of BS2 cells to HFO parti-
cles and after 10 days of growth (T10) showing HFO particles almost
completely reduced to soluble Fe(II). (f) Organic acid, H 2 and CO 2
amounts by strain BS2 (in mol.h−1) in glucose and glucose + HFO media
during the exponential and stationary/death phases (Adapted from
Lehours et al. 2010 )16 Methanogens and Methanotrophs in Lake Pavin