The Lotus japonicus Genome

(Steven Felgate) #1

Fungal pathogen,Fusarium solani, was also
reported as a model pathogen ofL. japonicus
(Takeuchi et al. 2007 ). A fungal isolate,
MAFF240020 (deposited in GenBank, National
Institute of Agrobiological Sciences), causes the
wilt disease by root rot inL. japonicusaccession
Gifu B-129. In addition, MAFF240020 also
causes chlorotic to necrotic lesions on leaves
after wound inoculation. Thus, F. solani
MAFF240020 is suitable for studying pathogenic
interactions inL. japonicus.


15.6 Conclusion


As discussed above, our knowledge about the
Lotusdefense mechanism largely relies on the
study of symbiosis and mainly restricted to MTI.
Even so, these studies revealed the presence of
close relationship between defense and symbiosis
and thus highlight strongly the importance of the
study ofLotus–pathogen interactions. Investiga-
tions of the defense mechanisms inL. japonicus,
especially about ETI, will contribute to the
understanding of not only legume defense
mechanisms but also the true nature of symbiosis.


References


Alippi A (2005) Bacterial diseases ofLotusspp. Lotus
Newslett 35(1):17– 18
Ausmees N, Kobayashi H, Deakin WJ, Marie C, Krishnan
HB, Broughton WJ, Perret X (2004) Characterization
of NopP, a type III secreted effector ofRhizobiumsp.
strain NGR234. J Bacteriol 186(14):4774– 4780
Bartsev AV, Deakin WJ, Boukli NM, McAlvin CB,
Stacey G, MalnoëP, Broughton WJ, Staehelin C
(2004) NopL, an effector protein ofRhizobiumsp.
NGR234, thwarts activation of plant defense reac-
tions. Plant Physiol 134(2):871– 879
Chen WM, Laevens S, Lee TM, Coenye T, De Vos P,
Mergeay M, Vandamme P (2001) Ralstonia taiwan-
ensis sp. nov., isolated from root nodules of Mimosa
species and sputum of a cysticfibrosis patient. Int J
Syst Evol Microbiol 51(Pt 5):1729– 1735
Christie PJ, Vogel JP (2000) Bacterial type IV secretion:
conjugation systems adapted to deliver effector mol-
ecules to host cells. Trends Microbiol 8(8):354– 360
Cullimore JV, Ranjeva R, Bono JJ (2001) Perception of
lipo-chitooligosaccharidic Nod factors in legumes.
Trends Plant Sci 6(1):24– 30


Deakin WJ, Broughton WJ (2009) Symbiotic use of
pathogenic strategies: rhizobial protein secretion sys-
tems. Nat Rev Microbiol 7(4):312– 320
Deakin WJ, Marie C, Saad MM, Krishnan HB, Broughton
WJ (2005) NopA is associated with cell surface
appendages produced by the type III secretion system
ofRhizobiumsp. strain NGR234. Mol Plant Microbe
Interact 18(5):499– 507
Dodds PN, Rathjen JP (2010) Plant immunity: towards an
integrated view of plant-pathogen interactions. Nat
Rev Genet 11(8):539– 548
Felix G, Duran JD, Volko S, Boller T (1999) Plants have
a sensitive perception system for the most conserved
domain of bacterialflagellin. Plant J 18(3):265– 276
Gomez-Gomez L, Boller T (2000) FLS2: an LRR
receptor-like kinase involved in the perception of the
bacterial elicitorflagellin inArabidopsis. Mol Cell 5
(6):1003– 1011
Graham PH, Vance CP (2003) Legumes: importance and
constraints to greater use. Plant Physiol 131
(3):872– 877
Hayashi M, Saeki Y, Haga M, Harada K, Kouchi H,
Umehara Y (2012) Rj (rj) genes involved in nitrogen-
fixing root nodule formation in soybean. Breed Sci 61
(5):544– 553
Heidrich K, Blanvillain-Baufume S, Parker JE (2012)
Molecular and spatial constraints on NB-LRR receptor
signaling. Curr Opin Plant Biol 15(4):385– 391
Hueck CJ (1998) Type III protein secretion systems in
bacterial pathogens of animals and plants. Microbiol
Mol Biol Rev 62(2):379– 433
Jones JD, Dangl JL (2006) The plant immune system.
Nature 444(7117):323– 329
Kambara K, Ardissone S, Kobayashi H, Saad MM,
Schumpp O, Broughton WJ, Deakin WJ (2009)
Rhizobia utilize pathogen-like effector proteins during
symbiosis. Mol Microbiol 71(1):92– 106
Kouchi H, Imaizumi-Anraku H, Hayashi M, Hakoyama
T, Nakagawa T, Umehara Y, Suganuma N, Kawag-
uchi M (2010) How many peas in a pod? Legume
genes responsible for mutualistic symbioses under-
ground. Plant Cell Physiol 51(9):1381– 1397
Krause A, Doerfel A, Göttfert M (2002) Mutational and
transcriptional analysis of the type III secretion system
ofBradyrhizobium japonicum. Mol Plant Microbe
Interact 15(12):1228– 1235
Lopez-Gomez M, Sandal N, Stougaard J, Boller T (2012)
Interplay offlg22-induced defence responses and
nodulation in Lotus japonicus. J Exp Bot 63
(1):393– 401
Marie C, Deakin WJ, Viprey V, Kopciñska J, Golinowski
W, Krishnan HB, Perret X, Broughton WJ (2003)
Characterization of Nops, nodulation outer proteins,
secreted via the type III secretion system of NGR234.
Mol Plant Microbe Interact 16(9):743– 751
Miya A, Albert P, Shinya T, Desaki Y, Ichimura K,
Shirasu K, Narusaka Y, Kawakami N, Kaku H,
Shibuya N (2007) CERK1, a LysM receptor kinase, is
essential for chitin elicitor signaling inArabidopsis.
Proc Natl Acad Sci U S A 104(49):19613– 19618

168 T. Nakagawa et al.

Free download pdf