The Lotus japonicus Genome

19

Wild Accessions and Mutant Resources

Masayoshi Kawaguchi and Niels Sandal

Abstract

Lotus japonicus,Lotus burttii,andLotusfilicaulisare species ofLotus

genus that are utilized for molecular genetic analysis such as the

construction of a linkage map and QTL analysis. Among them, a number

of mutants have been isolated from two wild accessions:L. japonicusGifu

B-129 and Miyakojima MG-20. Here, we show the wild accessions and a

list of all mutants isolated so far.

19.1 Wild Accessions and Isolated
Mutants

Lotus japonicus(Regel) Larsen is widely dis-
tributed in Eastern Asia, China, Korea, Taiwan,
and Japan (Grant et al. 1962 ).L. japonicuswild-
type accession Gifu B-129 is most frequently
used for genetic analysis and was originally col-
lected on a riverbank in Gifu, a prefecture located
in the Chubu region of central Japan. A number of
mutants have been isolated from Gifu as shown in
the next paragraph. Another accession Miyakojima
MG-20 is often used as a crossing partner with
Gifu to produce F2 mapping populations

(Hayashi et al. 2001 ). MG-20 is an earlyflower-

ing ecotype suitable for indoor cultivation and

genetic analysis (Kawaguchi 2000 ). From a

comparative anatomical study, it has been pro-

posed that MG-20 be described as a separate

species,Lotus miyakojimae(Kramina) (Barykina

and Kramina 2006 ). To allow map-based cloning

and QTL analysis, two related diploid species

LotusfilicaulisB-37, originating from Algeria,

and Lotus burttiiB-303, from West Pakistan,

have been used as a crossing partner (Grant et al.

1962 ; Borsos et al. 1972 ; Sandal et al. 2002 ;

Kawaguchi et al. 2005 ). Compared with MG-20

andL.filicaulis,L. burttiishows an intermediate

level of polymorphism with respect to Gifu

(Sandal et al. 2012 ).

These wild-type accessions, with the exception

ofL.filicaulis,are available from the National Bio

Resource Project (NBRP)LotusandGlycine.To

assist with GWAS analysis in the future, many

more wild accessions ofL. japonicuswill need to

be collected from all over the world.

In order to unveil characteristics of legumes at

a molecular level, a large number ofL. japonicus

mutants, including those created by transposon

M. Kawaguchi (&)
Division of Symbiotic Systems, National Institute for
Basic Biology, Okazaki, Aichi 444-8585, Japan
e-mail: [email protected]

N. Sandal
Centre for Carbohydrate Recognition and Signalling,
Department of Molecular Biology and Genetics,
Aarhus University, Gustav Wieds Vej 10,
8000 Aarhus C, Denmark

S. Tabata and J. Stougaard (eds.),The Lotus japonicus Genome, Compendium of Plant Genomes,
DOI 10.1007/978-3-662-44270-8_19,©Springer-Verlag Berlin Heidelberg 2014

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