insertion and chemical/irradiation mutagenesis,
have been generated by research groups around
the world. In Table19.1, we summarize the
current status of all mutant lines isolated by
forward genetic screens and TILLING analysis.
These lines are grouped into 3 categories: sym-
biotic mutants, developmental mutants affecting
symbiosis, and non-symbiotic mutants. This list
will be useful not only for research but also for
the maintenance of genetic resources of L.
japonicus.
References
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in plastidic glutamine synthetase: functional properties
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Bonfante P, Genre A, Faccio A et al (2000) TheLotus
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Groth M, Takeda N, Perry J et al (2010) NENA, aLotus
japonicushomolog of Sec13, is required for Rhizo-
dermal infection by Arbuscular Mycorrhiza Fungi and
Rhizobia but dispensable for cortical endosymbiotic
development. Plant Cell 22:2509– 2526
Groth M, Kosuta S, Gutjahr C et al (2013) TwoLotus
japonicussymbiosis mutants impaired at distinct steps
of arbuscule development. Plant J 75:117– 129
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plant genome overcomes the lack of a bacterial gene
for symbiotic nitrogenfixation. Nature 462:514– 517
Hakoyama T, Niimi K, Yamamoto T et al (2012a) The
integral membrane protein SEN1 is required for
symbiotic nitrogenfixation inLotus japonicusnod-
ules. Plant Cell Physiol 53:225– 236
Hakoyama T, Oi R, Hazuma K et al (2012b) The SNARE
Protein SYP71 expressed in vascular tissues is
involved in symbiotic nitrogen fixation in Lotus
japonicusnodules. Plant Physiol 160:897– 905
Hayashi M, Miyahara A, Sato S et al (2001) Construction
of a genetic linkage map of the model legumeLotus
japonicususing an intraspecific F2 population. DNA
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Heckmann AB, Lombardo F, Miwa H et al (2006) Lotus
japonicus nodulation requires two GRAS domain
regulators, one of which is functionally conserved in
a non-legume. Plant Physiol 142:1739– 1750Table 19.1 (continued)
Locus Allele Affected in References
GWD1 gwd1-1, 2 Starch degradation Vriet et al. ( 2010 )
GWD3 gwd3-1, 4 Starch degradation Vriet et al. ( 2010 )
PGI pgi1- 1 to 3 Starch synthesis Vriet et al. ( 2010 )
PGM1 pgm1- 3 to 5 Starch synthesis Vriet et al. ( 2010 )
Sus1 sus1- 1 Sucrose synthase Horst et al. ( 2007 )
Sus3 sus3- 1 Sucrose synthase Horst et al. ( 2007 )
t.s.temperature sensitive
Snf represents spontaneous nodule formation
aIndicates mutants derived fromL. japonicusMG-20
19 Wild Accessions and Mutant Resources 217