Fukai E, Dobrowolska AD, Madsen LH, Madsen EB,
Umehara Y, Kouchi H, Hirochika H, Stougaard J
(2008) Transposition of a 600 thousand-year-old LTR
retrotransposon in the model legumeLotus japonicus.
Plant Mol Biol 68:653– 663
Fukai E, Umehara Y, Sato S, Endo M, Kouchi H, Hayashi
M, Stougaard J, Hirochika H (2010) Derepression of the
plant ChromovirusLORE1induces germline transpo-
sition in regenerated plants. PLoS Genet 6:e1000868
Fukai E, Soyano T, Umehara Y, Nakayama S, Hirakawa
H, Tabata S, Sato S, Hayashi M (2012) Establishment
of aLotus japonicusgene tagging population using the
exon-targeting endogenous retrotransposonLORE1.
Plant J 69:720– 730
Gorinsek B, Gubensek F, Kordis D (2004) Evolutionary
genomics of chromoviruses in eukaryotes. Mol Biol
Evol 21:781– 798
Gowda MVC, Bhat RS, Motagi BN, Sujay V, Kumari V,
Sujatha B (2010) Association of high-frequency origin
of late leaf spot resistant mutants with AhMITE1
transposition in peanut. Plant Breed 129:567– 569
Gowda MVC, Bhat RS, Sujay V, Kusuma P, Varshaku-
mari BS, Varshney RK (2011) Characterization of
AhMITE1transposition and its association with the
mutational and evolutionary origin of botanical types
in peanut (Arachis spp.). Plant Syst Evol 291:153– 158
Hancock CN, Zhang F, Floyd K, Richardson AO,
Lafayette P, Tucker D, Wessler SR, Parrott WA
(2011) The rice miniature inverted repeat transposable
element mPing is an effective insertional mutagen in
soybean. Plant Physiol 157:552– 562
Imaizumi R, Sato S, Kameya N, Nakamura I, Nakamura
Y, Tabata S, Ayabe S, Aoki T (2005) Activation
tagging approach in a model legume,Lotus japonicus.
J Plant Res 118:391– 399
Kanazawa A, Liu B, Kong F, Arase S, Abe J (2009)
Adaptive evolution involving gene duplication and
insertion of a novelTy1/copia-like retrotransposon in
soybean. J Mol Evol 69:164– 175
Li X, Song Y, Century K, Straight S, Ronald P, Dong X,
Lassner M, Zhang Y (2001) A fast neutron deletion
mutagenesis-based reverse genetics system for plants.
Plant J 27:235– 242
Liu B, Kanazawa A, Matsumura H, Takahashi R, Harada
K, Abe J (2008) Genetic redundancy in soybean
photoresponses associated with duplication of the
phytochrome A gene. Genetics 180:995– 1007
Madsen LH, Fukai E, Radutoiu S, Yost CK, Sandal N,
Schauser L, Stougaard J (2005)LORE1, an active low-
copy-number TY3-gypsy retrotransposon family in the
model legumeLotus japonicus. Plant J 44:372– 381
Mathieu M, Winters EK, Kong F, Wan J, Wang S, Eckert
H, Luth D, Paz M, Donovan C, Zhang Z, Somers D,
Wang K, Nguyen H, Shoemaker RC, Stacey G,
Clemente T (2009) Establishment of a soybean
(Glycine maxMerr. L) transposon-based mutagenesis
repository. Planta 229:279– 289
McCallum CM, Comai L, Greene EA, Henikoff S (2000)
Targeted screening for induced mutations. Nat Bio-
technol 18:455– 457
Patel M, Jung S, Moore K, Powell G, Ainsworth C,
Abbott A (2004) High-oleate peanut mutants result
from aMITEinsertion into theFAD2gene. Theor
Appl Genet 108:1492– 1502
Perry JA, Wang TL, Welham TJ, Gardner S, Pike JM,
Yoshida S, Parniske M (2003) A TILLING reverse
genetics tool and a web-accessible collection of mutants of
the legumeLotus japonicus. Plant Physiol 131:866– 871
Pislariu CI, Murray JD, Wen J, Cosson V, Muni RR,
Wang M, Benedito VA, Andriankaja A, Cheng X,
Jerez IT, Mondy S, Zhang S, Taylor ME, Tadege M,
Ratet P, Mysore KS, Chen R, Udvardi MK (2012) A
Medicago truncatulaTobacco Retrotransposon inser-
tion mutant collection with defects in nodule devel-
opment and symbiotic nitrogenfixation. Plant Physiol
159:1686– 1699
Rakocevic A, Mondy S, Tirichine L, Cosson V, Brocard
L, Iantcheva A, Cayrel A, Devier B, Abu El-Heba
GA, Ratet P (2009)MERE1, a low-copy-number
copia-type retroelement in Medicago truncatula
active during tissue culture. Plant Physiol
151:1250– 1263
Reid JB, Ross JJ (2011) Mendel’s genes: toward a full
molecular characterization. Genetics 189:3– 10
Rogers C, Wen J, Chen R, Oldroyd G (2009) Deletion-
based reverse genetics inMedicago truncatula. Plant
Physiol 151:1077– 1086
Schauser L, Roussis A, Stiller J, Stougaard J (1999) A
plant regulator controlling development of symbiotic
root nodules. Nature 402:191– 195
Shirasawa K, Hirakawa H, Tabata S, Hasegawa M,
Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A,
Fujishiro T, Isobe S (2012) Characterization of active
miniature inverted-repeat transposable elements in the
peanut genome. Theor Appl Genet 124:1429– 1438
Tadege M, Wen J, He J, Tu H, Kwak Y, Eschstruth A,
Cayrel A, Endre G, Zhao PX, Chabaud M, Ratet P,
Mysore KS (2008) Large-scale insertional mutagene-
sis using the Tnt1 retrotransposon in the model legume
Medicago truncatula. Plant J 54:335– 347
Thykjaer T, Stiller J, Handberg K, Jones J, Stougaard J
(1995) The maize transposable element Ac is mobile in
the legumeLotus japonicus. Plant Mol Biol 27:981– 993
Triques K, Sturbois B, Gallais S, Dalmais M, Chauvin S,
Clepet C, Aubourg S, Rameau C, Caboche M,
Bendahmane A (2007) Characterization ofArabidop-
sis thalianamismatch specific endonucleases: appli-
cation to mutation discovery by TILLING in pea.
Plant J 51:1116– 1125
Urbanski DF, Malolepszy A, Stougaard J, Andersen SU
(2012) Genome-wideLORE1retrotransposon muta-
genesis and high-throughput insertion detection in
Lotus japonicus. Plant J 69:731– 741
Urbanski DF, Malolepszy A, Stougaard J, Andersen SU
(2013) High-throughput and targeted genotyping of
Lotus japonicusLORE1 insertion mutants. In: Rose
RJ (ed) Legume genomics: methods and protocols,
methods in molecular biology, vol 1069, pp 119–146.
doi:10.1007/978-1-62703-613-9_10226 E. Fukai et al.