22.9 cDNA, TAC, and BAC Clones
Sato et al. ( 2008 ) sequenced the entireL. japo-
nicusgenome using the“Miyakojima MG-20”
strain. Various material resources such as trans-
formation-competent artificial chromosomes
(TAC) (Asamizu et al. 2003 ; Kaneko et al. 2003 ;
Kato et al. 2003 ; Nakamura et al. 2002 ; Sato
et al. 2001 ), bacterial artificial chromosomes
(BAC) (Sato et al. 2007 , 2008 ), and cDNA
libraries (Asamizu et al. 2000 , 2004 ) were
developed during the genome sequencing pro-
jects, and the information was made available at
the “miyakogusa.jp” Web site or the “Lotus
japonicusEST Index” hosted by the Kazusa
DNA Institute (Table 22.2). These important
products of L. japonicus genome sequenceFig. 22.4 EMS-treated mutants derived fromL. japoni-
cus“Miyakojima MG-20.”The numbers in parentheses
indicate the line number of the mutant.a,j(212-003);
abnormalflower and many short roots;b,e,i(212-103)
abnormalflower, crooked pod and twisted stem;c(01-
0210 ) abnormalflower;d(212-596) indiscrete leaf bract;
f(01-0017) long hypocotyl and defective hypocotyl
elongation with red-light irradiation;g(207-008) narrow
leaf;h(210-088) small leaf tip;k(211-039) pale brown
seed coat;l(01-0196)dwarf plant;m(206-105) white
leaf;n(205-074) variegated leaf;o(01-0348) swollen
root hair;p(01-1066) short root hair;q(01-0640) reduced
number of root hair;r(207-034a) abnormal root shape
and shorter root hair. These mutant lines were developed
by T. Sakai, RIKEN (present address, Niigata University)22 The National BioResource Project in Japan 251