Mesorhizobium/index.html), a database con-
structed by the Kazusa DNA Research Institute.
The M. loti mutant STM 5 that contains an
inserted transposon in the 3-phosphoglycerate
dehydrogenase gene played an important role in
identifying the molecular basis for effective
symbiosis between M. loti and L. japonicus
(Thapanapongworakul et al. 2010 ).
22.13 Mesorhizobium lotiPlasmid
Clones
Recently, 4,196 Mesorhizobium loti plasmid
clones were made available at“LegumeBase”
(Table22.1). This resource, deposited by the
Kazusa DNA Research Institute, comprises of
clones that were used in the genome sequencing
project of M. loti (Kaneko et al. 2000 ).
Manageable sizes of genomic DNA ofM. loti
were cloned into a pUC plasmid. These DNA
clones were linked mutually with the Genome
browser in RhizoBase (http://genome.microbedb.
jp/rhizobase/), a database forRhizobiumDNA
hosted by the Kazusa DNA Institute (Table22.
2 ). In the Genome browser, users are able to
select the DNA clones of interest and compare
the DNA sequence of the clone with other loci or
domains.22.14 DatabasesWe have constructed a Web page for NBRP
LotusandGlycine“LegumeBase”(http://www.
legumebase.brc.miyazaki-u.ac.jp/) at our
resource center that is composed of two dat-
abases, the“Lotus japonicusdatabase” (http://Fig. 22.6 Maps ofLjubq1promoter-based binary vec-
tors (Maekawa et al. 2008 ). The binary vectors are
represented as linear segments from the left to right
borders. These constructs were based on a vector in which
the 535-bp fragment of Ljubq1 containing the 5′
untranslated region intron was cloned into pCAM-
BIA1300. The hygromycin resistance gene originated
from the backbone vector, pCAMBIA1300. Reprinted
and modified by permission of the author254 M. Hashiguchi and R. Akashi