The Lotus japonicus Genome

(Steven Felgate) #1

23


Legume andLotus japonicusDatabases

Hideki Hirakawa, Terry Mun, Shusei Sato,
and Stig U. Andersen

Abstract
Since the genome sequence ofLotus japonicus, a model plant of family
Fabaceae, was determined in 2008 (Sato et al. 2008 ), the genomes of other
members of the Fabaceae family, soybean (Glycine max) (Schmutz et al.
2010 ) andMedicago truncatula(Young et al. 2011 ), have been sequenced.
In this section, we introduce representative, publicly accessible online
resources related to plant materials, integrated databases containing legume
genome information, and databases for genome sequence and derived
marker information of legume species includingL. japonicus.

23.1 Database for Plant Materials


Legume Base (http://www.legumebase.brc.
miyazaki-u.ac.jp/top.jsp), aL. japonicusandG.
maxdatabase, is developed by the National Bio-
Resource Project (NBRP) (http://www.nbrp.jp)
for distribution of plant materials. Users can order


seeds ofL. japonicusandG. max, bulked M2
seeds ofL. japonicus, DNA, vector and rhizo-
bium, root culture systems (superroot), and EMS
M2 bulked seeds ofG. maxas plant materials.
The biological materials pertaining toL. japoni-
cus hitherto available through Legume Base
(http://www.legumebase.brc.miyazaki-u.ac.jp/
lotus/top/top.jsp) are: three experimental
strains, 108 wild accessions, 205 LjMG
recombinant inbred lines (RILs, those of
crosses between Miyakojima MG-20 and Gifu
B-129), 171 mutant lines (EMS-treated
mutants), 6,671 Mesorhizobium loti STM
mutant clones, 10,839 BAC clones of Mi-
yakojima MG-20, 16,656 TAC clones of Mi-
yakojima MG-20, 92,389 cDNA clones
(Miyakojima MG-20 and Gifu B-129), endog-
enous long-terminal repeat (LTR) retrotrans-
poson, and Lotus Retrotransposon 1 (LORE1)
insertion tag lines. Meanwhile, biological
materials related to G. max (http://www.
legumebase.brc.miyazaki-u.ac.jp/glycine/top/
top.jsp) are: 265 cultivars, 715 wild accessions,

H. Hirakawa (&)S. Sato
Kazusa DNA Research Institute, Kazusa-Kamatari
2-6-7, Kisarazu, Chiba 292-0818, Japan
e-mail: [email protected]


T. MunS.U. Andersen
Centre for Carbohydrate Recognition and Signalling,
Department of Molecular Biology and Genetics,
Aarhus University, Gustav Wieds Vej 10,
8000 Aarhus C, Denmark
e-mail: [email protected]


S. Sato
Graduate School of Life Sciences, Tohoku
University, Katahira 2-1-1, Aoba-Ku, Sendai
980-8577, Japan
e-mail: [email protected]


S. Tabata and J. Stougaard (eds.),The Lotus japonicus Genome, Compendium of Plant Genomes,
DOI 10.1007/978-3-662-44270-8_23,©Springer-Verlag Berlin Heidelberg 2014


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