URLAPI/wwwblast/). BLAST searches using
amino acid or nucleotide sequences against the
LORE1 flanking sequences enables users to
search for genes of interest for which our
resource has insertional mutants available.
Results returned from this search are tagged with
a unique BLAST header, which users can sub-
sequently query the LORE1 database with.
Usability Tools
To aid the user, we have included some tools to
improve the usability of the resource [accessible via
“Tools,”Fig.23.5(2)]. The sequence retrieval tool
(SeqRet), based on the wwwfastacmd project and
whose modified source code is publicly available
(https://github.com/terrymun/wwwfastacmd),
allows users to retrieve full sequences of queried
identifying information, such as BLAST headers,
against relevant databases. Meanwhile, the
sequence processor tool (SeqPro) uses RegEx
parsing to clean up data provided by users, such as
largeamountsofformattedinformationfromlegacy
NCBI blast output, allowing for easy extraction of
relevant data.
A list of commonly asked questions (http://
users-mb.au.dk/pmgrp/faq.php) is provided to
guide users who may have queries that have been
previously addressed [accessible via“Tools[
FAQ,”Fig.23.5(3)].
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