Biology Today — December 2017

SECTION - A

1. Name the technique developed by Kary Mullis. What is the
   objective of this technique?


2. What is the role of ethidium bromide during agarose gel
   electrophoresis?


3. The milk produced by the transgenic cow Rosie is better
   than natural cow milk. Why?


4. Why do DNA fragments move towards the anode during gel
   electrophoresis?


5. Golden rice is a transgenic variety of rice. What is its
   characteristic property?

SECTION - B

6. (a) How is an exonuclease functionally different from an
   endonuclease?
   (b) Give example of any two endonucleases other than Sal I.


7. (a) Illustrate the recognition sequence of Eco R I. Mention
   the term used for such sequences.
   (b) How does restriction endonuclease act on a DNA
   molecule?


8. What are shuttle vectors?


9. (a) Why are transgenic animals so called?

(b) Name the scientist who introduced the term vaccine.

What are edible vaccines?

OR

Name the transgenic variety of tomato. How it remains

fresh for longer period than normal variety?

10. How is insertional inactivation of an enzyme used as a
    selectable marker to differentiate recombinants from
    non-recombinants?
    SECTION - C


11. Refer to the given figure and answer the following questions.
    (a) Name the organism in which the vector is inserted to
    get the copies of the desired gene.
    (b) Mention the area labelled in the vector responsible for
    controlling the copy number of the inserted gene.
    (c) Name and explain the role of a selectable marker
    shown in the vector.

EcoRI

ClaI HindIII

PvuI

PstI

BamHI

SalI

PvuII

ampR

Ori

rop

pBR322

GENERAL INSTRUCTIONS

(i) All questions are compulsory.

(ii) This question paper consists of five sections A, B, C, D and E. Section A contains 5 questions of one mark each, Section B contains

5 questions of two marks each, Section C contains 12 questions of three marks each, Section D contains 1 question of VBQ type with

four marks and Section E contains 3 questions of five marks each.

(iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the

three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions.

(iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

Time Allowed : 3 hours Maximum Marks : 70

UNIT - IV

• Biotechnology : Principles and
  Processes


• Biotechnology and its Applications