Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)

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Contents

Introduction ...................................................................................................... 1
Stephan A. Pless and Christopher A. Ahern

Engineered Ionizable Side Chains .................................................................. 5
Gisela D. Cymes and Claudio Grosman

Cysteine Modification: Probing Channel Structure, Function
and Conformational Change ........................................................................... 25
Myles H. Akabas

Functional Site-Directed Fluorometry ........................................................... 55
Michael Priest and Francisco Bezanilla

Bioreactive Tethers ........................................................................................... 77
Karen Mruk and William R. Kobertz

Flipping the Photoswitch: Ion Channels Under Light Control ................... 101
Catherine K. McKenzie, Inmaculada Sanchez-Romero
and Harald Janovjak

Incorporation of Non-Canonical Amino Acids .............................................. 119
Lilia Leisle, Francis Valiyaveetil, Ryan A. Mehl
and Christopher A. Ahern

Index .................................................................................................................. 153