60 M. Priest and F. Bezanilla
Fig. 1 Basic steps in functional site-directed fluorometry. Schematic drawings not to scale. a
Molecular biology for the functional site-directed fluorometry technique. ( 1 ) A plasmid ( black
circles) is combined with two DNA primers ( blue and red) containing the mutation of interest (in
this case, a cysteine substituted at a site of interest whose codon is shown as a C in the figure) to