New Horizons in Insect Science Towards Sustainable Pest Management

Diapause-Related Gene Expression in Eggs of Multivoltine Bombyx mori L. Silkworm Races 191

Sl. no. Clone ID Size (bp) Putative identity Percent identity E-value Matched Expressed
Sequence Tag
clone ID
During non diapause
1 ND–8 257 Propanediol utilization protein 99 1e–98 ovS315D08f
2 ND–10 188 Predicted similar to CG7849-
PB (pseudouridine synthase)

99 3e–69 fner29d24r

3 ND–21 166 Rsf1 (remodelling and splicing
factor 1)

100 7e–61 ovS334D03f

4 ND–24 181 40S ribosomal protein S14 97 1e–68 wdS30543
5 ND–17 135 40S ribosomal protein S5 98 8e–19 wdV10964X
6 ND–54 230 Ubiquitin family protein 98 3e–84 fcaL-P08_pT_N24
7 ND–11 182 RNA binding 99 5e–66 fner3n16r
8 ND–60 193 Translationally controlled
tumor protein homolog

99 2e–70 wdV30789

9 ND–50 231 Similar to ribosomal protein
L23Ae

100 9e–78 fdpeP07_F_B21

10 ND–13 307 Taste receptor type 2 member
117

99 1e–87 fner3p04r

11 2ND–18 229 Polyubiquitin 4 UBQ4 99 1e–56 fner50g04r
12 2ND–6 405 Chitinase A precursor 99 e–117 FWDP32_FL5_O09
13 ND–55 169 Eef2protein (eukaryotic trans-
lation elongation factor)

100 2e–33 prgv0458

14 ND–1A 195 Nucleosome assembly protein 99 0.0 Ce-1048
15 ND–1B 463 Nascent protein 99 0.0 wdV30032
16 ND–64 133 B. mori profilin protein 99 0.0 wdV30234
17 ND–3 140 B. mori acyl-coenzyme A dehy-
drogenase (acade)

100 0.0 NM_001044207.1

Table 2 Non-diapause upregulated genes isolated by suppressive subtractive hybridization. Percent identities, E-value,
matched Expressed Sequence Tag clone ID retrieved from Silkbase database ( http://www.silkdb.org ) and GenBank
( http://www.ncbi.nih.gov/ )

Clone ID Size (bp) Putative identity Percent identity E-value Matched EST clone ID
During diapause
D–4 262 Heat shock cognate 71 kDa
protein

99 1e–100 BmNP26_FL5_N17

D–12 185 B. mori ribosomal protein L41 99 6e–90 NM_001042449.1
D–28 107 60S ribosomal protein L8 99 3e–36 wdV40936
D–64 129 60S ribosomal protein L18 100 6e–45 wdV30295
D–68 228 60S ribosomal protein L27a 100 e–110 ovS334G05f
D–9 160 Ribosomal protein L14 100 3e–40 ovS325C03f
2D–12 98 Predicted similar to
ENSANGP00000018877
(chitin metabolic process)

96 4e–12 fdpeP08_F_H14

2D–14 367 40S ribosomal protein S19 99 e–125 fprwP27_F_G11
D–20 213 ENSANGP00000001579
(negative regulation of
transcription)

100 1e–59 BmNP15_T7_H13

Table 3 Diapause upregulated genes isolated by suppressive subtractive hybridization. Percent identities, E-value,
matched EST clone ID retrieved from Silkbase database ( http://www.silkdb.org ) and GenBank ( http://www.ncbi.nih.
gov/ )