New Horizons in Insect Science Towards Sustainable Pest Management

Inducing Resistance in Sesamum Accessions Against ... 289

allowed to mate and lay eggs on the seedlings.
Neonate larvae were transferred to potted plants
using a fine camel hairbrush.

Screening M 1 Generation

Based on LD50 evaluation, a dose of 50 krad was
identified as the LD 50 value for physical mutant
while for the chemical mutagens EMS and DES,
0.5 and 0.06 % were identified as LD50, respec-
tively. Upon evaluating the first mutant genera-
tion for resistance against A. catalaunalis, sever-
al leaf and growth habit mutants were recovered.
Chlorophyll mutants were also observed wherein
mostly pale-green or yellowish green leaves were
seen. One mutant was found to have more vigor-
ous growth while another mutant plant had tripod

at single node, which is peculiar. Based on leaf,

flower and capsule damage, mutants of four ac-

cessions namely IVTS 2001-7, NIC-7875, NIC-

16278 and TMV-3 were rated resistant, while

four accessions were moderately resistant and

two were susceptible (Tables 6 , 7 ).

Screening M 2 Generation

Plants of M 2 generation of the four accessions

namely IVTS 2001-7, NIC-16278, KMR 63, and

TMV-3 were rated as resistant, while another

four accessions were moderately resistant and

two accessions were susceptible (Tables 8 , 9 ).

Hence, it may be concluded that to ascertain the

segregation of such desirable traits in the mutant

generations, concerted field evaluation needs to

Table 6 Resistance rating of M 1 generation of sesame accessions against A. catalaunalis (Physical mutation)
Name of the
accession

Leaf damage

(%)

Resistance

rating

Flower damage

(%)

Resistance

rating

Capsule damage

(%)

Resistance
rating
NIC 7875 3.1 (10.14) R 1.5 (7.03) R 1.6 (7.26) R
NIC 7908 2.5 (9.09) MR 3.0 (9.97) MR 2.5 (9.09) MR
IVTS 2001-7 1.7 (7.49) R 1.5 (7.03) R 1.0 (5.73) HR
NIC 16278 3.0 (9.97) R 2.0 (8.13) R 1.2 (6.28) R
NIC 17345 5.0 (12.92) MR 3.0 (9.97) MR 2.0 (8.13) MR
KMR-56 4.5 (12.24) MR 2.4 (8.91) MR 2.0 (8.13) MR
KMR-63 4.0 (11.53) MR 2.8 (9.63) MR 2.5 (9.09) MR
KMR-102 6.0 (14.17) MR 3.0 (9.97) MR 2.7 (9.45) MR
TMV-3 2.4 (8.91) R 2.0 (8.13) R 2.2 (8.52) R
SVPRI-1 (‘S’ check) 15.2 (22.46) HS 6.2 (14.41) HS 6.2 (14.41) HS
C.D. 1.05 0.92 0.86

Table 7 Resistance rating of M 1 generation of sesame accessions against A. catalaunalis (Chemical mutation)
Name of the
accTession

Leaf damage

(%)

Resistance

rating

Flower damage

(%)

Resistance

rating

Capsule damage

(%)

Resistance
rating
NIC 7875 3.4 (10.62) R 2.2 (8.53) R 3.7 (11.09) MR
NIC 7908 1.9 (7.92) R 3.6 (10.93) MR 2.0 (8.13) MR
IVTS 2001-7 1.4 (6.79) HR 1.8 (7.71) R 0.9 (5.44) HR
NIC 16278 3.6 (10.93) R 2.9 (9.80) MR 1.8 (7.71) R
NIC 17345 1.9 (7.92) R 3.2 (10.30) S 2.9 (9.80) MR
KMR-56 4.9 (12.79) MR 2.7 (9.46) R 3.1 (10.14) MR
KMR-63 2.1 (8.33) R 4.1 (11.68) R 1.8 (7.71) R
KMR-102 6.4 (14.65) S 2.9 (9.80) MR 2.5 (9.10) MR
TMV-3 2.1 (8.33) R 0.8 (5.13) HR 1.3 (6.55) R
SVPRI-1 (‘S’ check) 13.1 (21.22) HS 9.6 (18.05) HS 7.3 (15.68) HS
C.D. 0.98 0.67 0.38