Chitinase Expressed as an Inducible Trait in Pseudomonas aeruginosa Schröter P-15 81
was found a reliable methodology to obtain re-
producible results (Li et al. 2007 ; Ghanem et al.
2010 ; Dong et al. 2012 ). Pseudomonas is a po-
tential producer of chitinolytic and other bio-
control enzymes (Fallahzadeh et al. 2010 ). The
optimal concentration ranges of the three factors
were optimized using CCD in RSM. As a result,
a quadratic model was found to fit for chitinase
production and the optimal medium composi-
tion was determined as follows: crude glyc-
erol—47.70 ml/l, colloidal chitin—38.75 g/l,
peptone—25.59 g/l, and chitinase activity
reached 3.75 Uml−1 when compared with the
predicted value of 2.88 Uml−1. A successful and
significant improvement (46.48 %) in the pro-
duction of chitinase by P. aeriginosa p-15 was
accomplished using cheaper carbon source and
inducer. The optimized medium established in
this work might result in a significant reduc-
tion in the cost of medium constituents. These
preliminary results also suggested employing
crude glycerol in media optimization studies for
chitinase production. Future trends in biocontrol
research will unite fundamental biology with the
quest for solutions that will make biocontrol in-
tegral to the safe and wise management of every
agroecosystem.
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