- An enzyme is classified as EC 2.7.1.1. The digit 2
denotes
(a) Class number
(b) Hydroxyl group as an acceptor
(c) Phosphate group acceptor
(d) Sub class - Synthetases are enzymes used in the class
(a) Transferase (b) Lyases
(c) Hydrolases (d) Ligases - Dehydrogenases are the enzymes included in the
class
(a) Hydrolases (b) Transferases
(c) Oxidoreductases (d) Ligases - According to IUB system, Oxidoreductase has
been included in
(a) EC2 (b) EC
(c) EC4 (d) EC - Horse radish peroxidase enzyme is used in the
technique
(a) Western blotting (b) ELISA
(c) Immuno diffusion (d) PCR - Taq DNA Polymerase enzyme is used in the
technique
(a) Radio immune assay
(b) Restriction digestion
(c) PCR
(d) ELISA - Luciferase is used to
(a) To detect bacterial contamination of foods
(b) In estimation of glucose
(c) In light detection reaction
(d) In estimation of fats - c 2. d 3. b 4. b 5. b
- c 7. b 8. a 9. c 10. a
- b 12. c 13. c 14. b 15. c
- b 17. c 18. c 19. a 20. d
- c 22. d 23. b 24. c 25. a
(c) It is a binding site which is separate from
the active site, and affects the activity of an
enzyme when it is occupied by a ligand.
(d) It is a description of an active site which has
undergone an induced fit.
- Which of the following descriptions best describes
an induced fit?
(a) The process by which an active site alters
shape such that it is ready to accept a substrate
(b) The process by which the substrate.
adopts the correct binding conformation
before entering an active site.
(c) The process by which a substrate binds to
an active site and alters the shape of active
site.
(d) The process by which an active site alters
the shape of the substrate such that it can
adopt the necessary active conformation for
binding. - Some enzymes require the presence of a non-
protein substance if they are to catalyse a reaction.
Which of the following terms is the best general
term for such a substance?
(a) Prosthetic group (b) Cofactor
(c) Co-enzyme (d) Modulator - What term is used for a non-protein organic
molecule that is required by some enzymes in
order to catalyse a reaction on a substrate?
(a) Prosthetic group (b) Cofactor
(c) Co-enzyme (d) Modulator - Enzymes are
(a) Thermophile (b) Thermolabile
(c) Thermostable (d) Allof these - The coenzyme is
(a) Often a metal (b) Always a protein
(c) Often a vitamin (d) Always an inorganic
compound - Which of the following enzyme is not
proteinacuous in nature?
(a) Urease (b) Peptidase
(c) Ribozyme (d) Phosphatase