In: Aquaculture ISBN: 978 - 1 - 63485 - 563 - 1
Editor: Joanne G. Buchanan © 201 6 Nova Science Publishers, Inc.
Chapter 2
PHAGE THERAPY OF VIBRIO HARVEYI:
A CONCEPT OF PROTECTION
BACTERIAL CONTAMINATION IN
AQUACULTURE INDUSTRY
Tirasak Pasharawipas
Department of Microbiology, Faculty of Sciences,
Rangsit University, Pathumthani, ThailandABSTRACT
The concept of phage therapy to control bacterial infection and
contamination was conceived about a century ago. It has now been more
important to understand this approach since drug resistant bacteria have
become more common and development of new antibiotics has become
more difficult. This article will cover the fundamental knowledge of
phage infection in bacterial cell and extends it to phage therapy by using
a model of Vibrio-phage, called VH-P, with its specific Vibrio harveyi
host, VH 13- 1. The VH 13-1 was isolated and collected from luminescent
black tiger shrimp. To test the possibility of the use of permissive phage
as a tool to treat the VH contamination in shrimp ponds, a VH-P which is
a temperate phage was tested for its efficiency to kill the VH13-1. In the
meantime VH 16 - 1 which is not infected by VH-P was used as a control.
Our study was conducted with the MOI (multiple of infection) of 1-100.
The success of the treatment is determined if no VH bacterial colony is
observed in the culture medium after the phage treatment. The MOI of 20