structure was incubated in 37 °C to investigate the unfolding of electronics in
MatrigelTM.
Co-inject mesh electronics with cells: Hippocampal neurons were prepared
using a standard protocol described previously [ 4 ]. Cells were isolated by cen-
trifugation at 200 g for 1 min and then resuspended in 5–10 mg/ml MatrigelTMat
4 °C. MatrigelTMwith neurons were mixed with electronics at 4 °C and then loaded
into syringe with metal gauge needle. Mesh electronics and neurons were co-injected
into 30% (v/v) polymerized MatrigelTMin a culture plate and then placed in the
incubator to allow the MatrigelTMto cure at 37 °C for 20 min. Then 1.5 mL of
NeuroPure plating medium was added. After 1 day, the plating medium was
changed to NeurobasalTMmedium supplemented with B27, GlutamaxTMand 0.1%
Gentamicin reagent solution. The in vitro co-cultures were maintained at 37 °C with
5% CO 2 for 14 days, with medium changed every 4–6 days.
5.2.4 Injection of Mesh Electronics into Behaving Animals....
Animal preparation: Adult (25–35 g) male C57BL/6J mice (Jackson lab) were
group-housed, giving access to food pellets and water ad libitum and maintained on
Fig. 5.3 Loading in needle.
Schematics show how the
mesh electronics was stepwise
loaded into glass needle.
Blue, glass needle; pink,
plastic tube; yellow, injectable
electronics; black, I/O pads
and blue, nanowire devices.
aThe tip of glass needle was
connected to syringe by
plastic tube. Injectable
electronics was sucked in
from the end of glass needle.
bElectronics was loaded into
glass needle.cGlass needle
was mounted to patch-clamp
setup for injection
70 5 Syringe Injectable Electronics