115and T. timopheevii (Farshadfar et al. 1994 ; Mikó et al. 2014 ) and T. monococcum
(Megyeri 2014 ).
The crossability alleles were further transferred from the Mv9 kr1 genotype into
two modern Martonvásár wheat cultivars, Mv Magdaléna and Mv Béres (Molnár-
Láng et al. 2010 ). The main objective of this work was to develop a wheat genotype
containing both the recessive crossability alleles ( kr1kr1kr2kr2 ), all owing high
crossability between 6× wheat and diploid rye, and the 1BL.1RS wheat/ rye translo-
cation chromosome. This wheat genotype could be used as a recipient partner in
wheat-rye crosses for the effi cient introduction of new allelic variation into the 1RS
arm in translocation wheats. After the wheat cultivars Mv Magdaléna and Mv Béres,
in which the 1BL. 1RS translocation involves the 1RS chromosome arm from
Petkus, were crossed with line Mv9 kr1, 117 F 2 plants were analysed for crossabil-
ity, 10 of which had more than 50 % seed set with rye and thus presumably carried
the kr1kr1kr2kr2 alleles. Four of these contained the 1BL. 1RS translocation in the
disomic condition, as detected by genomic in situ hybridization (GISH). The aver-
age seed set with rye on selected F 3 progenies from the Mv Béres × Mv9 kr1 and Mv
Magdalena × Mv kr1 crosses was 65.5 % and 64.7 %, respectively (Fig. 4.3 ). On
some plants the seed set with rye was as high as 87.5 or 83.3 %. When the wheat × rye
F 1 hybrids produced between these lines and the rye cultivar Kriszta were analyzed
Fig. 4.3 In situ hybridization on mi totic chromosomes of a wheat-rye F 1 hybrid. Genomic in situ
hybridization (GISH) on somatic chromosomes of the wheat × rye hybrid produced between the
Mv Béres kr1 line and the rye cv. Kriszta. Total rye genomic DNA was labeled with digoxigenin
and detected with anti-dig-rhodamine. Seven rye chromosomes and the 1RS arm in the 1BL.1RS
translocated chromosome are magenta colour. Twenty wheat chromosomes and the 1BL arm of
the 1BL.1RS chromosome are blue as a result of counterstaining with DAPI
4 The Crossability of Wheat with Rye and Other Related Species