Fig. 12.6 Sequential GISH and FISH on mitotic chromosomes from 7DL.7DS-5HS wheat–barley
disomic translocation line. ( a ) Identifi cation of the chromosomes using DNA probes pSc119.2
( green ), Afa family ( red ) and pTa71 ( yellow ). ( b ) Barley total genomic DNA was labelled with
biotin and detected with streptavidin-FITC ( green ). Wheat chromosomes were counterstained with
DAPI ( blue )
barry
(Barry)
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