Alien Introgression in Wheat Cytogenetics, Molecular Biology, and Genomics

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Fig. 13.1 Molecular cytogenetic identifi cation of mitotic metaphase chromosomes in ( a ) T. aesti-
vum cv. Chinese Spring (2 n = 6 x = 42; BBAADD); ( b ) Ae. biuncialis MvGB382 (2 n = 4 x = 28;
U b U b M b M b ); ( c ) Ae. uniaristata JIC2120001 (2 n = 2 x = 14; NN); ( d ) Ae. comosa MvGB1039
(2n = 2x = 14; MM); and ( e ) Ae. umbellulata AE740/03 (2 n = 2 x = 14; UU). Fluorescence in situ
hybridization (FISH) was done using repetitive DNA probes for Afa family repeat (red), pSc119.2
repeat (green) and pTa71 repeat (yellow) and allowed identifi cation of all chromosomes in the
karyotypes. Scale bar = 10 μm

13 Genomics of Wild Relatives and Alien Introgressions