361Fig. 13.3 Mono- ( a ) and biparametric ( b ) fl ow cytometric analysis and sorting of mitotic meta-
phase chromosomes from Ae. umbellulata (2 n = 2 x = 14; UU). ( a ) Monoparametric analysis of
chromosomes stained by DAPI results in a histogram of relative fl uorescence intensity (fl ow
karyotype) in which three peaks representing chromosomes 1U, 6U and 3U are discriminated. The
remaining four chromosomes form a composite peak and cannot be sorted individually.
Biparametric analysis of chromosomes stained by DAPI and with GAA repeats labeled by FITC
results in a bivariate fl ow karyotype on which all seven chromosomes (colored regions) can be
discriminated and fl ow-sorted at a purity of 90–99 %
13 Genomics of Wild Relatives and Alien Introgressions