Orton, D.R. 1992. Quantitative and mechanistic effects of bubble aeration on animal cells in
culture. Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA.
Papoutsakis, E.T. (1991a) Fluid mechanical damage of animal cells in bioreactors. TIBTECH 9.
427–37.
Papoutsakis, E.T. (1991b) Media additives for protecting freely suspended animal cells against
agitation and aeration damage. TIBTECH 9, 316–324.
Petersen, J.F., Mclntire, L.V. and Papoutsakis, E.T. (1988) Shear sensitivity of cultured hybridoma
cells (CRL-8018) depends on mode of growth, culture age and metabolite concentration. J.
Biotechnol 7, 229–246.
Petersen, J.F., Mclntire, L.V. and Papoutsakis, E.T. (1990) Shear sensitivity of hybridoma cells in
batch, fed-batch, and continuous cultures. Biotechnol. Prog. 6, 114–120.
Pol, L. van der, Zijlstra, C.M., Thalen, M. and Tramper, J. (1990) Effect of serum concentration on
production of monoclonal antibodies and on shear sensitivity of a hybridoma. Bioproc. Eng. 5,
241–245.
Pol, L. van der, Bakker, W.A.M. and Tramper, J. (1992) Effects of low serum concentrations (0%–
2.5%) on growth, production, and shear sensitivity of hybridoma cells. Biotechnol. Bioeng. 40,
179–182.
Pol, L. van der, Bonarius, D., Wouw, G.van der, and Tramper, J. (1993) Effect of silicone antifoam
on shear sensitivity of hybridoma cells in sparged cultures. Biotechnol. Prog. 9, 504–509.
Ramirez, O.T. and Mutharasan, R. (1990) The role of the plasma-membrane fluidity on the shear
sensitivity of hybridomas grown under hydrodynamic stress. Biotechnol. Bioeng. 36, 911–920.
Ramirez, O.T. and Mutharasan, R. (1992) Effect of serum on the plasma-membrane fluidity of
hybridomas: An insight into its shear protective mechanism. Biotechnol. Prog. 8, 40–50.
Rooney, J.A. (1970) Hemolysis near an ultrasonically pulsating gas bubble. Science 169, 869–871.
Sato, M., Levesque, M.J. and Nerem, R.M. (1987) An application of the micropipette technique to
the measurement of the mechanical properties of cultured bovine aortic endothelial cells. J.
Biochem. Eng. 109, 27–34.
Schurch, U., Kramer, H.Einsele, A.Widmer, F. and Eppenberger, H.M. (1988). Experimental
evaluation of laminar shear stress on the behavior of hybridoma mass cell cultures, producing
monoclonal antibodies against mitochondrial creatine kinase. J. Biotechnol. 7, 179–184.
Smith, C.G., Greenfield, P.F. and Randerson, D.H. (1987) A technique for determining the shear
sensitivity of mammalian cells in suspension culture. Biotechnol. Technol. 1, 39–44.
Stathopoulos, A. and Heliums, J.D. (1985) Shear stress effects on human embryonic kidney cells in
vitro. Biotechnol. Bioeng. 27, 1021–1026.
Tan, W.S., Dai, G.C. and Chen, Y.L. (1995) Quantitative investigations of cell-bubble interactions
using a foam fractionation technique. Cytotechnol. 15, 321–328.
Telling, R.C. and Elsworth, R. (1965) Submerged culture of hamster kidney cells in a stainless steel
vessel. Biotechnol. Bioeng. 7, 417–434.
Thomas C.R., Al-Rubeai M. and Zhang, Z. (1995) Prediction of mechanical damage to animal cells
in turbulence. Cytotechnol. 15, 329–335.
Tramper, J., Williams, J.B. and Joustra, D. (1986) Shear sensitivity of insect cells in suspension.
Enzyme Microbiol Technol. 8, 33–36.
Tramper, J., Smit, D., Straatman, J. and Vlak, J.M. (1988) Bubble-column design for growth of
fragile insect cells. Bioproc. Eng. 3, 37–41.
Trinh, K., Garcia-Briones, M., Hink, F. and Charniers, J.J. (1994) Quantification of damage to
suspended insect cells as a result of bubble rupture. Biotechnol. Bioeng. 43, 37–45.
Wang, N.S., Yang, J.-D., Calabrese, R.V. and Chang, K.-C. (1994) Unified modeling framework of
cell death due to bubbles in agitated and sparged bioreactors. J. Biotechnol. 33, 107–122.
Wen, Z.Y. and Tan, W.S. (1999) Quantitative measurement of cell-bubble attachment in bubble
columns using foam flotation techniques. Biotechnol. Techn. 13, 131–135.
Wu, J. and Goosen, M.F.A. (1994) Investigation of air-liquid damage of animal cells in a falling
film-flow device. Biotechnol. Techn. 8, 111–116.
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