Cannabis sativa L. - Botany and Biotechnology

with 3 ppm 2,4,5-T. More biosynthesis studies in 1987 were conducted by Braemer and Paris ( 1987 ) for investigating the convers ...

13.2.5 Regeneration Efficient plant regeneration protocol is essential for mass production of pharma- ceutically superior elite ...

acid into a medium containing 0.5lM thidiazuron slightly increased shoot growth. Elongated shoots when transferred to half-stren ...

shoot cultures were encapsulated in calcium alginate beads. The best gel com- plexation was achieved using 5% sodium alginate wi ...

Chaohua C, Gonggu Z, Lining Z, Chunsheng G, Qing T, Jianhua C, Xinbo G, Dingxiang P, Jianguang S (2016) A rapid shoot regenerati ...

Lata H, Chandra S, Mehmedic Z, Khan IA, ElSohly MA (2012)In vitrogermplasm conservation of high delta (9)-tetrahydrocannabinol y ...

Small E, Marcus D (2002) Tetrahydrocannabinol levels in hemp (Cannabis sativa) germplasm resources. Econ Bot 57:545– 558 Toivone ...

Chapter 14 Hairy Root Culture as a Biotechnological Tool inC. sativa Imane Wahby, Juan Manuel Caba and Francisco Ligero Abstract ...

(35% by weight, polyunsaturated fatty acid rich) is of nutritional and industrial value (Callaway 2004 ; Li et al. 2010 ) and ch ...

response in many plant species further leading to the idea thatrolgenes might act synergistically. This natural phenomenon could ...

Hairy roots can also serve as starting material to regenerate transgenic plants. Transformed roots from a number of plant specie ...

fluorescent lamps). Thus, hemp seedlings of healthy appearance are obtained, appropriate for in vivo (plantlets) or in vitro (ex ...

typical of hairy root: three days post-infection, wounded hypocotyl areas became swollen, 2–3 days later small calli started to ...

14 Hairy Root Culture as a Biotechnological Tool inC. sativa 305 ...

tumor growth, suggesting some differential response to Agrobacteriumwithin C. sativagermplasm. Finally, tumors could be cultured ...

staining (Fig.14.1f), demonstrating cotransfer of the binary vector and the expression of the foreign GUS gene. All this provide ...

tetrehydrocannabinolic acid (THCA) synthase has recently been cloned fromC. sativaplants and its heterologous expression in toba ...

Department of Analytical Chemistry, University of Granada) (Wahby et al. 2006 ). With this methodology, atropine, choline and mu ...

transformed root cultures together with elicitation approaches (Mehrotra et al. 2015 ) as systems for biotechnological productio ...

3% sucrose), supplemented with different combinations of BA and kinetin (Kin) as cytokinins and indole-3-acetic acid (IAA), ANA ...