Cannabis sativa L. - Botany and Biotechnology

Chapter 19 Assessing Genetic Diversity inCannabis sativaUsing Molecular Approaches Zamir K. Punja, Gina Rodriguez and Sarah Chen ...

19.1 Introduction The cannabis plant (Cannabis sativaL.), a member of the family Cannabaceae, has been cultivated by humans sinc ...

During the 1960s and 1970s, plant breeders began collecting landrace strains and often referred to them as heirloom varieties. T ...

(8 weeks) and that all could be triggered by providing a 12 h night duration which is the period of darkness that is critical to ...

19.4 Mating Systems in Cannabis Cannabis sativais an obligately outbreeding species under natural conditions and is dioecious (m ...

cannabis plants, it is reported by marihuana producers that hermaphrodites can occur. Usually, hermaphrodites are plants that be ...

1979 ). In a female line of cucumber, silver nitrate application (0.02–0.03%) increased the frequency of maleflowers and hermaph ...

distinguished by their unique names e.g.‘Special Kush’,‘Jesus OG’,‘Congolese Red’,‘Girl Scout Cookies’, etc. Many of these strai ...

19.7 Random Amplified Polymorphic DNA (RAPD) Analysis of Cannabis Several early studies have demonstrated the applicability of R ...

Kayis et al. ( 2010 ) demonstrated that both methods provided similar results with respect to identifying genetic diversity and ...

Among the landraces studied,‘Brazil Amazonia’,‘Columbia Gold’,‘Hoa Bac Silver’, and‘Kilamanjaro’, originated from Brazil, Columb ...

Fig. 19.4 Flower bud development of four landraces grown under similar conditions—“Brazil Amazonia (upper left),‘Columbia Gold ( ...

Fig. 19.5 Dendrogram showing the genetic relationship among landrace and other strains of cannabis originating from diverse geog ...

Fig. 19.7 Banding patterns obtained using ISSR primer UBC 817 following PCR of DNA from 8 landraces of cannabis (labeled attop) ...

Fig. 19.9 Dendrogram showing the genetic relationship among 10 strains of cannabis used in commercial production and originating ...

(Fig.19.11). The results also show there is a considerable level of genetic diversity among strains of marihuana developed for c ...

19.10 Short Tandem Repeat (STR) and SSR Analysis Hsieh et al. ( 2003 ) investigated the use of short tandem repeat loci in ident ...

19.11 Sequence Characterized Amplified Regions (SCAR) Analysis of Cannabis The use of SCAR markers has been primarily for the de ...

(500 and 730 bp in size) which were detected in all male plants but were absent in female plants. These two DNA fragments were c ...

common and widely used strains for production by seed companies would allow for accurate investigations of genetic diversity wit ...