was hydrolyzed producing impure compounds (Wollner et al. 1942 ). In 1960, de
Ropp, described the isolation ofD^9 -THC ( 1 ) from theflowering tops ofC. sativa.
Adsorption chromatography of the methanolic extract of cannabis followed by
partition chromatography on Celite using N, N-dimethyl formamide/cychlohexane
mixture and high vacuum distillation to getD^9 -THC. But the determination ofD^9 -
THC purity was based only on thin layer chromatography (de Ropp 1960 ). Thefirst
isolation of the naturally occurringD^9 -THC ( 1 ) in its pure form was reported by
Fig. 7.5 Cannabinoids other thanΔ^9 -THC-type ( 82 – 104 )
7 Natural Cannabinoids of Cannabis and Methods of Analysis 171