Table 13.1In vitro protocols developed forCannabis sativaL.ExplantResponseMediumReferenceSeedling partsCell suspension culturesModified Gamborg’s mediumVeliky and Genest (1972)Root, hypocotyl, leaves of seedling, male andfemalefloral partsCallus culturesMS + 0.1- 0.01 ppm
KIN + 1.0 ppm 2,4-DItokawa et al. (1975)Seedling partsCell suspension culturesItokawa et al. (1977)Embryo, leaf, stemCallus and cell suspension culturesMS + 3 mg/l 2,4,5-TLoh et al. (1983)Seedling partsCell suspension culturesMS + B5 vitamins + 3 mg/l2,4,5-THartsel et al. (1983)LeafCell suspension culturesB5 + 0.5 mg/l KIN + 1 mg/l 2,4 -DBraemer et al. (1987)AnthersCell suspension cultures: cryopreservation10% DMSOJekkel et al. (1989)LeafCell cultureMS + B5 vitamins + 1 mg/l2,4-D + 1 mg/l KINFlores-Sanchez et al.(^2009)LeafCallus cultureMS + 0.5μM NAA + 1.0μMTDZLata et al. (2010)InternodesCallus cultureMS + 1 mg/l BAP + 0.5 mg/lNAAJiang et al. (2015)CotyledonCallus cultureMS + 2 mg/l TDZ + 0.5 mg/LIBAMovahedi et al. (2015)Stem and leaf segment from seedlingCallus culture; Agrobacterium mediatedtransformationMS + B5 vitamins + 5μM 2,4D+1μM KINFeeney and Punja (2003)HypocotylHairy root culturesA. rhizogenes and A. tumefaciensstrainsWahby et al. (2006,^2013)SeedlingHairy root cultures from callusB5 + 4 mg/l NAAFarag and Kayser (2015)Root development from callusHemphill et al. (1978)Root development from callusFisse et al. (1981)Stem, cotyledon, rootCallus formationMS + NAAFisse and Andres (1985)(continued)13 Micropropagation ofCannabis sativaL.—An Update 289