Systems Biology (Methods in Molecular Biology)

tumors in nude mice. Indeed, MCF-7 are widely recognized to

present only some malignant features, showing a low aggressive

behavior [94, 95]. The cell line HTB-126 shows a much greater

value for the entropy production rate compared to a MCF7 (see

Table3).

The human hepatoma cells HepG2 and Hep3B have very

different morphology and even differ in their growth rates.

HepG2 cells are slowly growing with average doubling time around

48 h whereas Hep3B grows faster with doubling time of 24 h.

These cell lines differ inp53 functionality/mutation. Hep3B is

p53-null, while HepG2 has wild-type. Also, Hep3B contain

Table 3
Entropy production rate for different human tumor cells

Human tumor cells

Doubling time

[90] (h)

Respiration rate

[88]min mg cellnmolO^2



Si     10 ^9

kcal

Kminmg cell



HeLa (human cervix carcinoma) 48 3–6.5 0.102

MCF-7 (human breast carcinoma) 60 [91] 7 0.150

HTB-126 (human breast carcinoma) 40 [91] 28.5 0.611

SH-SY5Y (human neuroblastoma) > 55 10 0.215

SF188 (human glioblastoma) – 5.5 0.118

H460 (human lung cancer cells) 42–60 6 0.129

HCC4017 (non-small-cell lung

cancer)

• 12.4 0.266

HL-60 (human promyelocytic

leukemia cells)

About 40 2–3.4 0.058

U937 (human histocytic leukemia

cells)

30–40 1.5–3.3 0.052

Jurkat (human acute lymphoblastic

leukemia cells)

25–35 3.6 0.077

KMS20 (human myeloma) – 30 0.644

HepG2 (human hepatoma cells) 50–60 6.7 0.144

Hep3B (human hepatoma cells) 40–50 9.6 0.206

LNCAP (human prostate cancer) About 60 18.7 0.401

PC3 (human prostate cancer) 50 45 0.965

143B (human osteosarcoma) – 4.9 0.105

AGS (human gastric cancer cell line) 20 [92] 8 0.172

For doubling time the reference is [90], except for those whose reference is either individually given in doubling time
column or no value at all is given. Reprinted from [22]

Parameters Estimation in Phase-Space Landscape Reconstruction of Cell Fate... 147