multiplied CCD camera. Biophys J
95:5385–5398- Di Rienzo C, Gratton E, Beltram F et al (2013)
Fast spatiotemporal correlation spectroscopy
to determine protein lateral diffusion laws in
live cell membranes. Proc Natl Acad Sci U S A
110:12307–12312 - Schwille P, Haupts U, Maiti S et al (1999)
Molecular dynamics in living cells observed by
fluorescence correlation spectroscopy with
one- and two-photon excitation. Biophys J
77:2251–2265 - Weiss M, Hashimoto H, Nilsson T (2003)
Anomalous protein diffusion in living cells as
seen by fluorescence correlation spectroscopy.
Biophys J 84:4043–4052 - Wawrezinieck L, Rigneault H, Marguet D et al
(2005) Fluorescence correlation spectroscopy
diffusion laws to probe the submicron cell
membrane organization. Biophys J
89:4029–4042 - Lenne PF, Wawrezinieck L, Conchonaud F
et al (2006) Dynamic molecular confinement
in the plasma membrane by microdomains and
the cytoskeleton meshwork. EMBO J
25:3245–3256 - Hell SW, Wichmann J (1994) Breaking the
diffraction resolution limit by stimulated emis-
sion: stimulated-emission-depletion fluores-
cence microscopy. Opt Lett 19:780–782 - Eggeling C, Ringemann C, Medda R et al
(2009) Direct observation of the nanoscale
dynamics of membrane lipids in a living cell.
Nature 457:1159–1162 - Mueller V, Ringemann C, Honigmann A et al
(2011) STED nanoscopy reveals molecular
details of cholesterol- and cytoskeleton-
modulated lipid interactions in living cells. Bio-
phys J 101:1651–1660 - Sezgin E, Levental I, Grzybek M et al (2012)
Partitioning, diffusion, and ligand binding of
raft lipid analogs in model and cellular plasma
membranes. Biochim Biophys Acta
1818:1777–1784 - Hebert B, Costantino S, Wiseman PW (2005)
Spatiotemporal image correlation spectroscopy
(STICS) theory, verification, and application to
protein velocity mapping in living CHO cells.
Biophys J 88:3601–3614- Sako Y, Kusumi A (1995) Barriers for lateral
diffusion of transferrin receptor in the plasma
membrane as characterized by receptor drag-
ging by laser tweezers: fence versus tether. J
Cell Biol 129:1559–1574 - Sako Y, Kusumi A (1994) Compartmentalized
structure of the plasma membrane for receptor
movements as revealed by a nanometer-level
motion analysis. J Cell Biol 125:1251–1264 - Di Rienzo C, Gratton E, Beltram F et al (2014)
From fast fluorescence imaging to molecular
diffusion law on live cell membranes in a com-
mercial microscope. J Vis Exp 9:e51994 - Ries J, Chiantia S, Schwille P (2009) Accurate
determination of membrane dynamics with
line-scan FCS. Biophys J 96:1999–2008 - Ritchie K, Shan XY, Kondo J et al (2005)
Detection of non-Brownian diffusion in the
cell membrane in single molecule tracking.
Biophys J 88:2266–2277 - Di Rienzo C, Gratton E, Beltram F et al (2016)
Spatiotemporal fluctuation analysis: a powerful
tool for the future nanoscopy of molecular
processes. Biophys J 111:679–685 - Kolin DL, Wiseman PW (2007) Advances in
image correlation spectroscopy: measuring
number densities, aggregation states, and
dynamics of fluorescently labeled macromole-
cules in cells. Cell Biochem Biophys
49:141–164 - Digman MA, Brown CM, Sengupta P et al
(2005) Measuring fast dynamics in solutions
and cells with a laser scanning microscope. Bio-
phys J 89:1317–1327 - Di Rienzo C, Gratton E, Beltram F et al (2016)
Super-resolution in a standard microscope:
from fast fluorescence imaging to molecular
diffusion laws in live cells. In: Alberto D, AMJ
v Z (eds) Super-resolution imaging in biomed-
icine. Taylor & Francis Group, Abingdon, pp
19–47 - Di Rienzo C, Piazza V, Gratton E et al (2014)
Probing short-range protein Brownian motion
in the cytoplasm of living cells. Nat Commun
5:5891
290 Francesco Cardarelli