22 A Coculture-Based Approach for Screening Campaigns Aimed
at Identifying NovelPseudomonas aeruginosaQuorum
Sensing Inhibitors. ..................................................... 287
Giordano Rampioni, Giulia Giallonardi, Francesca D’Angelo,
and Livia Leoni
23 A Culture-Dependent Method for the Identification of Quorum
Quenching Enzymes of Microbial Origin.................................. 297
Kaihao Tang and Xiao-Hua Zhang
24 Directed Evolution of Quorum-Quenching Enzymes: A Method
for the Construction of a Directed Evolution Platform
and Characterization of a Quorum-Quenching Lactonase
fromGeobacillus kaustophilus............................................. 311
Maybelle Kho Go, Jeng Yeong Chow, and Wen Shan Yew
25 Generation of High-Sensitivity Monoclonal Antibodies Specific
for Homoserine Lactones.. .............................................. 325
Soumya Palliyil
26 Identification of AI-2 Quorum Sensing Inhibitors inVibrio harveyi
Through Structure-Based Virtual Screening. ............................... 353
Tianyu Jiang, Peng Zhu, Lupei Du, and Minyong Li
27 Identification of Staphylococcal Quorum Sensing Inhibitors
by Quantification of o ̃-Hemolysin with High Performance
Liquid Chromatography................................................. 363
Cassandra L. Quave and Alexander R. Horswill
Index.. .................................................................... 371
Contents xi