Quorum Sensing

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leukocytes is quorum-sensing dependent.
Microbiology 151:373–383


  1. Høiby N, Krogh Johansen H, Moser C, Song
    Z, Ciofu O, Kharazmi A (2001)Pseudomonas
    aeruginosaand thein vitroandin vivobiofilm
    mode of growth. Microbes Infect 3:23–35

  2. Pamp SJ, Gjermansen M, Johansen HK,
    Tolker-Nielsen T (2008) Tolerance to the
    antimicrobial peptide colistin inPseudomonas
    aeruginosabiofilms is linked to metabolically
    active cells, and depends on the pmr and
    mexAB-oprM genes. Mol Microbiol 68:
    223–240

  3. Passador L, Cook JM, Gambello MJ, Rust L,
    Iglewski BH (1993) Expression ofPseudomo-
    nas aeruginosavirulence genes requires cell-to-
    cell communication. Science 260:1127–1130

  4. Pearson JP, Passador L, Iglewski BH, Green-
    berg EP (1995) A secondN-acylhomoserine
    lactone signal produced byPseudomonas aeru-
    ginosa. Proc Natl Acad Sci U S A
    92:1490–1494

  5. Diggle SP, Winzer K, Chhabra SR, Worrall KE,
    Ca ́mara M, Williams P (2003) ThePseudomo-
    nas aeruginosaquinolone signal molecule over-
    comes the cell density-dependency of the
    quorum sensing hierarchy, regulatesrhl-depen-
    dent genes at the onset of stationary phase and
    can be produced in the absence of LasR. Mol
    Microbiol 50:29–43

  6. Hentzer M, Givskov M (2003) Pharmacologi-
    cal inhibition of quorum sensing for the treat-
    ment of chronic bacterial infections. J Clin
    Invest 112:1300–1307

  7. Hentzer M, Riedel K, Rasmussen TB, Heydorn
    A, Andersen JB, Parsek MR et al (2002) Inhibi-
    tion of quorum sensing inPseudomonas aerugi-
    nosabiofilm bacteria by a halogenated furanone
    compound. Microbiology 148:87–102

  8. Wu H, Song Z, Hentzer M, Andersen JB,
    Heydorn A, Mathee K et al (2000) Detection
    ofN-acylhomoserine lactones in lung tissues of
    mice infected with Pseudomonas aeruginosa.
    Microbiology 146:2481–2493

  9. Wu H, Song Z, Hentzer M, Andersen JB,
    Molin S, Givskov M et al (2004) Synthetic
    furanones inhibit quorum-sensing and enhance
    bacterial clearance inPseudomonas aeruginosa
    lung infection in mice. J Antimicrob Che-
    mother 53:1054–1061

  10. Andersen JB, Heydorn A, Hentzer M, Eberl L,
    Geisenberger O, Christensen BB et al (2001)


gfp-based N-acyl homoserine-lactone sensor
systems for detection of bacterial communica-
tion. Appl Environ Microbiol 67:575–585


  1. Andersen JB, Sternberg C, Poulsen LK, Bjorn
    SP, Givskov M, Molin S (1998) New unstable
    variants of green fluorescent protein for studies
    of transient gene expression in bacteria. Appl
    Environ Microbiol 64:2240–2246

  2. Hentzer M, Wu H, Andersen JB, Riedel K,
    Rasmussen TB, Bagge N et al (2003) Attenua-
    tion ofPseudomonas aeruginosavirulence by
    quorum sensing inhibitors. EMBO J
    22:3803–3815

  3. Kohler T, van Delden C, Curty LK, Hamzeh-
    pour MM, Pechere JC (2001) Overexpression
    of the MexEF-OprN multidrug efflux system
    affects cell-to-cell signaling in Pseudomonas
    aeruginosa. J Bacteriol 183:5213–5222

  4. van Gennip M, Christensen LD, Alhede M,
    Phipps R, Jensen PØ, Christophersen L et al
    (2009) Inactivation of therhlAgene inPseudo-
    monas aeruginosaprevents rhamnolipid pro-
    duction, disabling the protection against
    polymorphonuclear leukocytes. APMIS
    117:537–546

  5. Pedersen SS, Shand GH, Hansen BL, Hansen
    GN (1990) Induction of experimental chronic
    Pseudomonas aeruginosalung infection withP.
    aeruginosaentrapped in alginate microspheres.
    APMIS 98:203–211

  6. Bjarnsholt T, van Gennip M, Jakobsen TH,
    Christensen LD, Jensen PØ, Givskov M
    (2010)In vitroscreens for quorum sensing
    inhibitors and in vivo confirmation of their
    effect. Nat Protoc 5:282–293

  7. Moser C, Johansen HK, Song Z, Hougen HP,
    Rygaard J, Høiby N (1997) ChronicPseudo-
    monas aeruginosalung infection is more severe
    in Th2 responding BALB/c mice compared to
    Th1 responding C3H/HeN mice. APMIS
    105:838–842

  8. Chalfie M, Tu Y, Euskirchen G, Ward WW,
    Prasher DC (1994) Green fluorescent protein
    as a marker for gene expression. Science
    263:802–805

  9. Cormack BP, Valdivia RH, Falkow S (1996)
    FACS-optimized mutants of the green fluores-
    cent protein (GFP). Gene 173:33–38

  10. Høiby N (1974) Epidemiological investiga-
    tions of the respiratory tract bacteriology in
    patients with cystic fibrosis. Acta Pathol Micro-
    biol Scand B Microbiol Immunol 82:541–550


212 Louise Dahl Hultqvist et al.

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