3 Methods
3.1 Burn and
Infection Model
3.1.1 Bacterial Inoculum
Preparation
- StreakP. aeruginosaPA14 cells from a 80 C stock onto LB
agar plate and incubate the plates at 37C overnight. - The next day, inoculate one bacterial culture tube containing
5 ml of LB-Lennox liquid medium with one isolated colony
and incubate at 37 C, rotating at 200 rpm, until an
OD600nm¼1.0 is reached.
Fig. 1Burn and infection model. Materials used in this model (a–c). Mice are shaved (d,e), depilated (f,g),
given a full thickness skin burn injury (h–j), infected (k), and put back in their cages (l)
Animal Models for Anti-Virulence Therapies 231